LOCUS       sequence-68459-e        7527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length P12 ectodomain 
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: P12-bio
FEATURES             Location/Qualifiers
     source          1..7527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2907..2962
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2961..2980)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5198..5216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5284..5388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5389..5457,5458..6249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5607..5626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6420..7008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6909..6928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7162..7179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7296..7317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7332..7337,7338..7355,7356..7362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7370..7386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7375..7397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7394..7410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7394..7410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccaca agaacctggc ctgcgacttc aacgacgtgt
     1381 acaagctgga attccacccc aaccagcaga ccagcgtgac caagctgtgc aacctggccc
     1441 ccaacgtgct ggaaaaagtg accatcaagt gcggcagcga caagctgaac tacaacctgt
     1501 acccccccac ctgtttcgag gaagtgtacg ccagccggaa catgatgcac ctgaagaaga
     1561 tcaaagagtt cgtcatcggc agcagcatgt tcatgcggcg gagcctgacc cccaacaaga
     1621 tcaacgaggt gtccttccgg atccccccca acatgatgcc cgagaagccc atctactgct
     1681 tttgcgagaa caagaaaacc atcaccatca acggcgccaa cggcaacccc agcagcaaga
     1741 aggacatcat caaccggggc atcgtggaga tcatcatccc cagcctgaac gagaaagtga
     1801 agggctgcga tttcaccacc tccgagagca ccatcttcag caagggctac agcatcaacg
     1861 agatcagcaa caaggccagc aacaaccagc aggacatcgt ctgcaccgtg aaggcccacg
     1921 ccaacgacct gatcggcttc aagtgcccca gcaactacgc cgtggagccc cacgattgct
     1981 tcgtgagcgc cttcaacctg gccggcaaga acgagaacct ggaaaacaag ctgaagctga
     2041 ccaacatcat catggaccac tacaacaacg ccttctactc tcgcctgccc agcctgatca
     2101 gcgacaactg gaagttcttc tgcgtgtgca gcaaggacaa cgagaagaaa ctggtgttca
     2161 ccgtggaggc cagcatcagc agcagcaaca ccaagctggc tagccgggac aacacctacc
     2221 aggactacat cagcaacagc ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     2281 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     2341 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     2401 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     2461 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     2521 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     2581 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     2641 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     2701 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     2761 agatggactc caagatccag gttttatcca aagggttgaa ttccggatca ctgcatcata
     2821 ttctggatgc acagaaaatg gtgtggaatc atcgttaagt ctagacacgt gattaattaa
     2881 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2941 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     3001 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     3061 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     3121 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     3181 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     3241 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     3301 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     3361 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     3421 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     3481 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     3541 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     3601 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     3661 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     3721 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3781 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3841 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3901 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3961 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     4021 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     4081 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     4141 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     4201 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     4261 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     4321 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     4381 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     4441 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     4501 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     4561 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4621 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4681 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     4741 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4801 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4861 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4921 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4981 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5041 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5101 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     5161 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5221 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5341 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5461 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5521 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5581 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     5641 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5761 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5821 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5881 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5941 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6061 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7261 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7321 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7381 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     7441 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     7501 tgcaggcgca gaactggtag gtatgga
//
