LOCUS       sequence-68461-e        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length Pf92 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: Pf92-bio
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4245..4300
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4299..4318)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6536..6554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6622..6726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6727..6795,6796..7587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6945..6964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7758..8346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8247..8266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8500..8517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8634..8655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8670..8675,8676..8693,8694..8700)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8708..8724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8713..8735
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8732..8748
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8732..8748
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaacg tggccttcga gcagaaggtg cagaccaacc
     1381 tggcccacga caagaacggc gacatcgtgt acggccaccg ggagttcaag ggcggcatct
     1441 acgcctttct gggctacgac aactgcgcca tcgaagtgaa caaggccgtg aacggcatcg
     1501 actggaacga gaagaaagac gtgaaggtgt ccggcaacaa caatatcgcc gtggtgtaca
     1561 gcatcttcac cagcgaggaa aagatgatcc tgatcttcaa gtgcgacaac aagttctaca
     1621 tcaccaagta cggcaaagaa ttcaagtgga gcgaccccaa agtgatcgac gtgtccaacg
     1681 tgatcggcac caacaccgcc cctgctgtgt acagcggaag cctgctgtcc atgaacaacg
     1741 acttcgagaa gtacatcctc gtgtgcgaga accacgccca gaactacatc aacgtggtgg
     1801 accaagaata catgcgggag atcagactgc tgggaaagtg catgctgtcc ttcgacgagg
     1861 gcaacaactg gaagaacgaa gtgatgaacc tgtacagcga cgagggctac accaagatca
     1921 acaccctgcg gctgtccgac tacggcggca agatcctggt caagggcacc aacgcccaga
     1981 atctgaacca ggccatccgg tccatcatcc tgctgtgcag caatctgcac gactggaagc
     2041 tgttctgcgg cctgcccacc atccggttcc ggaaggacat cagcgtggag aacctggcct
     2101 acctgaacac ctaccacctg gccatcgtga agaacgagga caagctgcag ctggccttca
     2161 cctacgacct gttcgagaca ttcgaccccc agtacctgaa taccgagttc aacggcgtga
     2221 gccactactt cgtgctggcc cccgacgaga tggtgtacct gttctaccac ggcaatgaga
     2281 agaaaaacta cgtgatcaag atcaagaccg tgccccggaa gatcggctgc gagctgaaca
     2341 ccaacgacgc cgtgaacaag atctacacct acacatacaa gtacatctac aacaacaagc
     2401 tgtccgccaa gacatgcaag gtgcccagca gccacctgaa gtacagcagc gacggcctgt
     2461 acaagctgtt cgaagtgcgg ctgcccaagg acatcaaagt gaccgagaac tgcttccggt
     2521 acagcttcct gagcgacctg aacaacaagt accacaccac catcatcaag accagagtga
     2581 tcaataagct ggaagattac gtggaggtgc agttccactt ccccatctac tacaccaagt
     2641 tcctctacaa ctacaagagc acctactgcg tgctgtccaa caactaccgg atcgtggtgg
     2701 agttcgacta catccggaac cacatcgacc tggacttccc tttcgacacc gacaccgtga
     2761 agctgtacag caatgaggcc gtgacccacg ccttccggaa caacgccgag aaaacccacg
     2821 tgcacaagtt ccccaagggc acctacatga ccagctactt cagctacgag aaagaatacg
     2881 tgatctccaa ctacatcgag gaacccttca gcaccacctt caccatcctg acccagaccc
     2941 agatgaacgt gcacttcatg gctggcggcc agaagtacaa gtacgagggc atcgacctga
     3001 ccgacagcag ccccaactac gagctgtccc tgaacagcct gagcgacagc cagaacgtgg
     3061 acatcttcgt gagcaagttc gacaataaca aggccatcgg cttcgtgtgc cccgtgaagt
     3121 cctcctacga cggcctgaac tgcttcgaca acgtgtacat caagaacaag gccctggtca
     3181 agatcgagta cctgttcggc gagaacgaca tctttgtggt gcctcagcgg cggatctaca
     3241 agaccgaggg caccgccatg gaatccctgc tgtatctgaa caataacaac gtgaagaagc
     3301 tgatcgacga caagagcatc atccacttct actgcgagtg caacgtgaac aacaacgtga
     3361 tcaaagtgaa ctactacatc agccccttct acgacgagaa cagcatcaag caggaaatca
     3421 acaagaagga ccaagaaatc accatgatca acaaagccat cccccaggac gagaaggata
     3481 tcctgttcaa caacgagaaa gtggtgcccc tgagcaacga gcctcaggaa atcatccagc
     3541 cccccatcca ggaaaagctg aataccgccg accccagcgg cgcgccgtcg acctccatca
     3601 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     3661 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     3721 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     3781 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     3841 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     3901 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     3961 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     4021 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     4081 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     4141 ccggatcact gcatcatatt ctggatgcac agaaaatggt gtggaatcat cgttaagtct
     4201 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     4261 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4321 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     4381 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     4441 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     4501 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     4561 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     4621 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     4681 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     4741 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     4801 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     4861 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     4921 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     4981 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     5041 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     5101 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     5161 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     5221 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     5281 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     5341 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     5401 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     5461 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     5521 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     5581 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     5641 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     5701 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     5761 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     5821 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     5881 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     5941 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     6001 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     6061 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6121 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     6181 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     6241 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     6301 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     6361 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     6421 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     6481 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     6541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6961 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7261 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8581 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8641 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8701 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     8761 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     8821 caacgttgtt gccattgctg caggcgcaga actggtaggt atgga
//
