LOCUS       sequence-68462-e        9393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length Pf113 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: Pf113-bio
FEATURES             Location/Qualifiers
     source          1..9393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4773..4828
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4827..4846)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7064..7082)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7150..7254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7255..7323,7324..8115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7473..7492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8286..8874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8775..8794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9028..9045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9162..9183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9198..9203,9204..9221,9222..9228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9236..9252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9241..9263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9260..9276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9260..9276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcctacg tgcacaacga cgtgatcaag ttcggcgaag
     1381 aaaacagcct gaagtgcagc cagggcaacc tgtacgtgct gcactgcgag gtgcagtgcc
     1441 tgaacggcaa caacgagatc atccacaagc ggtgcaacga cgacatcgag aagaagtgca
     1501 acggcaataa caagtgcatc tactttttcg agtacgagct gcggaagaaa acccagagct
     1561 tccggaacaa gaacagcatc gagatcagcg agtgcgtgga gagcgagcag aacgaagtga
     1621 aaaccagcac cacctgtctg ctgtccaaca gcttcatcct ggacgaggcc ttcatccagt
     1681 actttttctt catcaagaac aagaacgagg aacccgtgat ctgcaaggac ggcaacatca
     1741 acatcaagag cgccctgctg cacagcccct tttgcgagat caagctgaag gacatcagcg
     1801 agtacatccg gaagaagtgc gacaacaaca aagagtgcct gatcgacccc ctggacgtgc
     1861 agaagaatct gctgaacgaa gaggacccct gctacatcaa caacgcctac gtgtccgtga
     1921 acgtcgtgtg caacaaagag gaagagatcg gcgacgagag caccgacagc agcagcatgg
     1981 aaatccagga cagcaccagc aacgagcagg acgagaacgt gaagggcatg agcagcagcc
     2041 aggaaatgaa ctccaacaac gacgagaaca agaatcagga caacgaggcc gacgacgacg
     2101 tgaacaacaa caataacaat aataacgacg accaggacga gcagggcaac gatggcgacg
     2161 tgaccagcag catgaataag aatgaggaca ataaggacct ggaacacggc agcagcaacg
     2221 atgtgaacaa taacgccgac accctggtca ataacaaaga gaacaaagaa ttcgtcctga
     2281 aagagaagtc cagcctgacc agcaagatca acaaagagct ggcccaccgg accgccctgt
     2341 tcaacaagct ggccgacaac attgccctgc tgctgaacaa gaagtacgac tccttcgaga
     2401 tcaaggacgt gctggaagat cggtacaacg agatgaagcg ggacgccaac cccgacgtgt
     2461 actacatcta cctgatggac accctggata tcgagaagat cgaggacatc aacctggaag
     2521 aggtcaagat gagcctgctc gcctctctga aagaaaccat gaacaagatc gacaccatcg
     2581 agaaaaagat cgaagagttc aagaataagt acatcagcct gtacaacaaa gtgaaaacaa
     2641 ccatgcccga gctgttcgac ctgaacgagg acctggtgct gctgtacaac gacttcccct
     2701 tcgacaacgg catgatcagc tccgacatct tcttcaagta caaccccagc gagaacatca
     2761 tggaccacca ggaaatggtc aagaagggca gcatcaccga ggacgagctg agaatcgtga
     2821 acgacctgga acccctggac aactaccggc ggagaaagcg gatcaccgag ctgcgcaaga
     2881 tcctggtgga gaagctgcgg atcctgtacc tggaaaagaa caacctgttc aatacccagg
     2941 ccagctgcat caagagctac tgctacaaga accccctgaa cctgaaaacc ctggaagtgc
     3001 tgctgaagaa aaactactac cggctgaaag aaaacaagga ctacgacgtg gtgtccagca
     3061 tcatccagca tctcgacaac gtggacgcca acaagaaaaa gaagtggctg acccacgagc
     3121 ggatcctgaa gaaactccag gtgctgatcg ccgagggcta caagagaatc aacgagaaag
     3181 agaaggacat cgaccggcgg atggccgtgt acaacgccct gtacgagaag gcccagagct
     3241 acaatctgca gaagctgttc aacgacgcca acgactttct gaagaaatac gccatcatgg
     3301 gcaacagctt cgacgacggc gacgaggtgt tcggcagcca gagcagcaac ttcaacatct
     3361 tcgacagcaa caacgccgac cagaacaatg agcaggaaca gcccaagcag gacgaccagc
     3421 tgctgaataa caacaacgat gacgtgctgt ccgagagcaa caacgaaaac aaagaaaaga
     3481 ccagcgacga cgccacccac aaagagacac aggaaaagag cgaccaggaa ccctcccaga
     3541 acatccagga agataacagc gacgagaagc acgccgagaa cgaagagaac gtggagcaga
     3601 tcgagacaga cagcaacgtg gccgaggaag ccaacgacga aaataaggac aacatgcaga
     3661 ccaccaccga tgagggcacc gaggaactgc agcagaacga cgaggatgct gagtctctga
     3721 ctaaagagaa ctccaagagc gaggaacagg aaaacgagga ctccaccgac gccgaggcca
     3781 tcgacaaaga agaggtggag acagaagaga agggcaagga cgaacagaag aaagacgagc
     3841 agaaagaaca ggacgaggaa gaggacggcg agaaagaaaa taagcacaag agcagcgaga
     3901 caaccaatga ggccgtgacc gacatcgagg aaaataagaa cgaggtcaag ggcgaagaac
     3961 atctgcaggg cagcgagcag agcatcgagg ccagcgagag cagccagaag gacgagacaa
     4021 aagagactga ggataaagag gaatacgtca acgccaatga cgacgagtcc agcgaagagg
     4081 acaccacccc caacgaggcc aacaagggcg cgccgtcgac ctccatcacg gcctataaga
     4141 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     4201 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     4261 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     4321 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     4381 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     4441 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     4501 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     4561 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     4621 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggatcactgc
     4681 atcatattct ggatgcacag aaaatggtgt ggaatcatcg ttaagtctag acacgtgatt
     4741 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     4801 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     4861 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     4921 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     4981 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     5041 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     5101 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     5161 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     5221 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     5281 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     5341 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     5401 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     5461 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     5521 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     5581 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     5641 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     5701 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     5761 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     5821 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     5881 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     5941 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     6001 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     6061 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     6121 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     6181 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     6241 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     6301 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     6361 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     6421 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     6481 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     6541 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     6601 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     6661 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     6721 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     6781 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     6841 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     6901 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     6961 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     7021 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     7081 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7141 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7201 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7261 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7321 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7381 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7441 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     7501 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7561 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7621 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7681 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7741 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     7801 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7861 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7921 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7981 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8041 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8101 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8401 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8881 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9061 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9181 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9241 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     9301 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     9361 cattgctgca ggcgcagaac tggtaggtat gga
//
