LOCUS       sequence-68468-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses neomycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Stewart Lab Plasmids
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 neo
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(452..470)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
       61 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      121 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
      181 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
      241 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
      301 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
      361 agcgtgaagc ttgatccggt ggatccccct ggggagagag gtcggtgatt cggtcaacga
      421 gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca
      481 ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc
      541 tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg
      601 ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg
      661 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag
      721 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg
      781 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag
//
