LOCUS       sequence-68482-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  guide RNA (gRNA) expression vector used to create a gRNA to a 
            specific sequence, uses U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Joung JK
  TITLE     Joung lab unpublished CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MLM3636
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..152
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        230..305
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(489..508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catcgatcag gctgttgaga gataattaga attaatttga ctgtaaacac aaagatatta
       61 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      121 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      181 ttatatatct tgtggaaagg acgaaacacc gagacgatta atgcgtctcg ttttagagct
      241 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      301 ggtgcttttt ttaagcttgg gccgctcgag gtacctctct acatatgaca tgtgagcaaa
      361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      841 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      961 caagcagca
//
