LOCUS       sequence-68484-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection, V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX304
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             365..670
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     549..568
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt
       61 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
      121 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
      181 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
      241 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
      301 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
      361 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
      421 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
      481 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
      541 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
      601 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
      661 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      721 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      781 tattgatatt tatatcattt tacgtttctc gttcagcttt ctttgtacaa agtggttggt
      841 aagcctatcc ctaacccctc t
//
