LOCUS       sequence-68487-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or
            MEFs by IMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX62
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..39
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     945..965
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctga
       61 agcttccgac ttcttggata cttgtaactt tgcgctttcc caccctcgca taagtttcct
      121 gaaataatga ctctgaaact caaaatatat ttacaaacac ctcggctgta gctttggttt
      181 gttctctgac ttgcttataa cttaatatcc ctcttattct aacctaagtt ctgccacgtg
      241 gttggttacc tctgctcagc ccccggcttc tgtcctccat gttcctgggg gaatccctac
      301 actttcagaa tttaatttcc ctactggatg tcccaccttc tttttattct accctttcct
      361 ataagccata ggggtttgtt tgtttgtatg ttttttaatt gacaagttat gcatccatac
      421 agtacacaat ctcttctctc tacagatggg caccgagtac aagcccacgg tgcgcctcgc
      481 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
      541 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
      601 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
      661 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
      721 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
      781 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
      841 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
      901 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
      961 cgagcggctc ggcttcaccg tcaccgc
//
