LOCUS       sequence-68489-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or
            MEFs by IMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX62
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    185..254
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc gccgacgccg
       61 tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc gagggcttcc
      121 cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc atcatgatgg
      181 accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc tggcacccgc
      241 accgcggctt cgagaccgtg acctacatcg tctctagtgc ggccgcacgg cattttgagt
      301 gttagactgg aaactttcca cttgataaga ggtcccaaga cttagtacct gaagggtgaa
      361 atattctcca tccagtggtt tcttctttgg ctggggagag gagctggtgt tgttgggcag
      421 tgctaggaaa gaggcaagga aaggtgataa aagtgaatct gaggcataac tgcacccttg
      481 gcctcctcca ccgcttcttg tcctgcttgc ttacctcgct tagtgctccc tgggggcagc
      541 tcgtggtgag gctccccttt cttgcggaga ttctcttcct ctgtgcgccg gtctctccca
      601 ggacaggcac aaacacgcac ctcaaagctg ttccgtccca gtagattacc actactcagg
      661 ataggaaaag agaagcaaga ggcagtaagg aaatcaggtc ctacctgtcc catttaaaaa
      721 accagactcc atctactccc aaccaccctt gtcctttctg gagcctaagc tccagctcca
      781 ggtaggtgga ggagaagcca caggttaaga ggtcccaaag ccagagaaaa gaaaactgag
      841 tgggagcagt aaggagattc cccgccgggg atgtgatgag aggtggatgg gtagtagtat
      901 ggaagaaatc ggtaagaggt gggcccaggg gtca
//
