LOCUS       sequence-6849-ex        3630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral construct for expressing human EGFR with L747-E749 
            deletion and A750P mutation in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, 
            Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
  TITLE     Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR 
            Mutants.
  JOURNAL   PLoS Med. 2005 Oct 4. 2(11):e313.
  PUBMED    16187797
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Med. 
            2005 Oct 4. 2(11):e313."
COMMENT     Sequence Label: EGFR (del3) L747-E749del, A750P
FEATURES             Location/Qualifiers
     source          1..3630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(4..75,76..1938,1939..2007,2008..3627)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKEPTSPKAN
                     KEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLL
                     NWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGG
                     KVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERL
                     PQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSP
                     TDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACID
                     RNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVY
                     HNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDN
                     PDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
ORIGIN
        1 actatgcgac cctccgggac ggccggggca gcgctcctgg cgctgctggc tgcgctctgc
       61 ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag gcacgagtaa caagctcacg
      121 cagttgggca cttttgaaga tcattttctc agcctccaga ggatgttcaa taactgtgag
      181 gtggtccttg ggaatttgga aattacctat gtgcagagga attatgatct ttccttctta
      241 aagaccatcc aggaggtggc tggttatgtc ctcattgccc tcaacacagt ggagcgaatt
      301 cctttggaaa acctgcagat catcagagga aatatgtact acgaaaattc ctatgcctta
      361 gcagtcttat ctaactatga tgcaaataaa accggactga aggagctgcc catgagaaat
      421 ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca accctgccct gtgcaacgtg
      481 gagagcatcc agtggcggga catagtcagc agtgactttc tcagcaacat gtcgatggac
      541 ttccagaacc acctgggcag ctgccaaaag tgtgatccaa gctgtcccaa tgggagctgc
      601 tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc
      661 tccgggcgct gccgtggcaa gtcccccagt gactgctgcc acaaccagtg tgctgcaggc
      721 tgcacaggcc cccgggagag cgactgcctg gtctgccgca aattccgaga cgaagccacg
      781 tgcaaggaca cctgcccccc actcatgctc tacaacccca ccacgtacca gatggatgtg
      841 aaccccgagg gcaaatacag ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat
      901 gtggtgacag atcacggctc gtgcgtccga gcctgtgggg ccgacagcta tgagatggag
      961 gaagacggcg tccgcaagtg taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga
     1021 ataggtattg gtgaatttaa agactcactc tccataaatg ctacgaatat taaacacttc
     1081 aaaaactgca cctccatcag tggcgatctc cacatcctgc cggtggcatt taggggtgac
     1141 tccttcacac atactcctcc tctggatcca caggaactgg atattctgaa aaccgtaaag
     1201 gaaatcacag ggtttttgct gattcaggct tggcctgaaa acaggacgga cctccatgcc
     1261 tttgagaacc tagaaatcat acgcggcagg accaagcaac atggtcagtt ttctcttgca
     1321 gtcgtcagcc tgaacataac atccttggga ttacgctccc tcaaggagat aagtgatgga
     1381 gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa
     1441 ctgtttggga cctccggtca gaaaaccaaa attataagca acagaggtga aaacagctgc
     1501 aaggccacag gccaggtctg ccatgccttg tgctcccccg agggctgctg gggcccggag
     1561 cccagggact gcgtctcttg ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc
     1621 aaccttctgg agggtgagcc aagggagttt gtggagaact ctgagtgcat acagtgccac
     1681 ccagagtgcc tgcctcaggc catgaacatc acctgcacag gacggggacc agacaactgt
     1741 atccagtgtg cccactacat tgacggcccc cactgcgtca agacctgccc ggcaggagtc
     1801 atgggagaaa acaacaccct ggtctggaag tacgcagacg ccggccatgt gtgccacctg
     1861 tgccatccaa actgcaccta cggatgcact gggccaggtc ttgaaggctg tccaacgaat
     1921 gggcctaaga tcccgtccat cgccactggg atggtggggg ccctcctctt gctgctggtg
     1981 gtggccctgg ggatcggcct cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg
     2041 cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg agaagctccc
     2101 aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa agtgctgggc
     2161 tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga gaaagttaaa
     2221 attcccgtcg ctatcaagga accaacatct ccgaaagcca acaaggaaat cctcgatgaa
     2281 gcctacgtga tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc
     2341 acctccaccg tgcaactcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc
     2401 cgggaacaca aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca
     2461 aagggcatga actacttgga ggaccgtcgc ttggtgcacc gcgacctggc agccaggaac
     2521 gtactggtga aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg
     2581 ggtgcggaag agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca
     2641 ttggaatcaa ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg
     2701 accgtttggg agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag
     2761 atctcctcca tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat
     2821 gtctacatga tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt
     2881 gagttgatca tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag
     2941 ggggatgaaa gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg
     3001 gatgaagaag acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc
     3061 ttcttcagca gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc
     3121 aacaattcca ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa
     3181 gacagcttct tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata
     3241 gacgacacct tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct
     3301 ggctctgtgc agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac
     3361 ccacactacc aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc
     3421 cagcccacct gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc
     3481 caccaaatta gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag
     3541 ccaaatggca tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca
     3601 caaagcagtg aatttattgg agcatgagat
//
