LOCUS       sequence-68492-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or
            MEFs by IMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX62
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             99..152
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgggagcg
       61 gcggtggctc tggtggcggt tcaatggtga ggctgctcga gggcagagga agtcttctaa
      121 catgcggtga cgtggaggag aatcccggcc ctgcaccggg agccaccatg actgccatgg
      181 aggagtcaca gtcggatatc agcctcgagc tccctctgag ccaggagaca ttttcaggct
      241 tatggaaact gtgagtggat ctttttgggg cccttaagat acatcccgcc atacctgtat
      301 cctccccttg cctgagagaa acaaaaacag tagtgttcaa acatggtatg gtgttgggtg
      361 tctgtaaatc ctgcggggcg gggtggcggg ggggggggac tgcagggtct cagaagtttg
      421 aggtcatcat tgactacata gcaagttgga ggccagcctg ggataagtga gattctgtct
      481 tcaaaaaatg gaaggaaatc aggaactaac tctctgctct tgttttccag acttcctcca
      541 gaagatatcc tggtaaggcc cagaacagaa agggacttgg gctttggtgg atcctgagga
      601 cctggtcctc tgactgctct tttcacccat ctacagtccc ccttgccgtc ccaagcaatg
      661 gatgatttga tgctgtcccc ggacgatatt gaacaatggt tcactgaaga cccaggtcca
      721 gatgaagctc ccagaatgcc agaggctgct ccccccgtgg cccctgcacc agcagctcct
      781 acaccggcgg cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag
      841 aaaacctacc agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaa
//
