LOCUS       sequence-68497-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral Gateway destination vector. Blasticidin 
            selection, V5 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pLX304
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..504)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(67..85)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(679..723)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc
       61 cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc
      121 acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca
      181 gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc
      241 gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa
      301 ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag
      361 ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg
      421 gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc
      481 agggctgcct tggaaaaggc gcaagggtcc cctcggggtt gggaggtggg tctgaaacga
      541 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
      601 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
      661 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
      721 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
      781 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
      841 actccaacta gcattccaag gcacagcagt ggtgcaaatg a
//
