LOCUS       sequence-68508-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or 
            MEFs by IMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX68
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..146
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtgcccgaag gacgcgcacc tggtgcatga cccgcaagcc cggtgccggg agcggcggtg
       61 gctctggtgg cggttcaatg gtgaggctgc tcgagggcag aggaagtctt ctaacatgcg
      121 gtgacgtgga ggagaatccc ggccctgcac cgggagccac catgactgcc atggaggagt
      181 cacagtcgga tatcagcctc gagctccctc tgagccagga gacattttca ggcttatgga
      241 aactgtgagt ggatcttttt ggggccctta agatacatcc cgccatacct gtatcctccc
      301 cttgcctgag agaaacaaaa acagtagtgt tcaaacatgg tatggtgttg ggtgtctgta
      361 aatcctgcgg ggcggggtgg cggggggggg ggactgcagg gtctcagaag tttgaggtca
      421 tcattgacta catagcaagt tggaggccag cctgggataa gtgagattct gtcttcaaaa
      481 aatggaagga aatcaggaac taactctctg ctcttgtttt ccagacttcc tccagaagat
      541 atcctggtaa ggcccagaac agaaagggac ttgggctttg gtggatcctg aggacctggt
      601 cctctgactg ctcttttcac ccatctacag tcccccttgc cgtcccaagc aatggatgat
      661 ttgatgctgt ccccggacga tattgaacaa tggttcactg aagacccagg tccagatgaa
      721 gctcccagaa tgccagaggc tgctcccccc gtggcccctg caccagcagc tcctacaccg
      781 gcggcccctg caccagcccc ctcctggccc ctgtcatctt ctgtcccttc ccagaaaacc
      841 taccagggca gctacggttt ccgtctgg
//
