LOCUS       sequence-68509-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or 
            MEFs by IMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX68
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    188..257
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 atcccgccgt gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg
       61 ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct
      121 tcccggtgcg ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga
      181 tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc
      241 cgcaccgcgg cttcgagacc gtgacctaca tcgtctctag tgcggccgca cggcattttg
      301 agtgttagac tggaaacttt ccacttgata agaggtccca agacttagta cctgaagggt
      361 gaaatattct ccatccagtg gtttcttctt tggctgggga gaggagctgg tgttgttggg
      421 cagtgctagg aaagaggcaa ggaaaggtga taaaagtgaa tctgaggcat aactgcaccc
      481 ttggcctcct ccaccgcttc ttgtcctgct tgcttacctc gcttagtgct ccctgggggc
      541 agctcgtggt gaggctcccc tttcttgcgg agattctctt cctctgtgcg ccggtctctc
      601 ccaggacagg cacaaacacg cacctcaaag ctgttccgtc ccagtagatt accactactc
      661 aggataggaa aagagaagca agaggcagta aggaaatcag gtcctacctg tcccatttaa
      721 aaaaccagac tccatctact cccaaccacc cttgtccttt ctggagccta agctccagct
      781 ccaggtaggt ggaggagaag ccacaggtta agaggtccca aagccagaga aaagaaaact
      841 gagtgggagc agtaaggaga ttccc
//
