LOCUS       sequence-68532-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-GAL4-hsp70
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(202..546)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 aatatattct atttattaac caagtagcta atatagctaa aatcaatttg ttgctaactt
       61 aaaactaaca taattatgta ttagttattt acattctttt ggttggttac aaccaaataa
      121 ataatatcac ttttaaaaac ttaagccagg aactgaataa ttttctattt ggctttagtc
      181 gacggtatcg atagacgtct attactcttt ttttgggttt ggtggggtat cttcatcatc
      241 gaatagatag ttatatacat catccattgt agtggtatta aacatccctg tagtgattcc
      301 aaacgcgtta tacgcagttt ggtccgtcca accaggtgac agtggttttg aattattacc
      361 atcatcaatt ttactagccg tgatttcatt attcatgaag ttatcatgaa cgttagagga
      421 ggcaattggt tgtgaaagcg cttgagaatt tgtttgagtt gttatgaggt tcggaccgtt
      481 gctactgtta gtgaaagtga aggacaatga gctatcagca atattcccac tttgattaaa
      541 attggcgcca ccaaacaaag cagacggggt cagtggcact aatgattgca gctgttgctg
      601 ttgccctaga aaaggcgtga ctgagcgatg cgaaggtgtg cttcttggta ttgtcactgg
      661 agagttacga gagggtggac ggttagataa cagcttgact agatcactga aacttgctcc
      721 tgatttcaat ggcacaggtg aaggccctac tgagccagga gaaacatatt taacactgat
      781 attgttgaca ttttcctccg gaagagtagg gtattgggcg atagttgcag aaccgacaat
      841 atttttaatg gcgctaccat tactattgtt ataactgata tgcgg
//
