LOCUS       sequence-68536-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Mukhtarov M, Liguori L, Waseem T, Rocca F, Buldakova S, Arosio D, 
            Bregestovski P
  TITLE     Calibration and functional analysis of three genetically encoded 
            Cl(-)/pH sensors.
  JOURNAL   Front Mol Neurosci. 2013 Apr 18;6:9. doi: 10.3389/fnmol.2013.00009. 
            Print 2013.
  PUBMED    23616745
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-04-18"; volume: "6:9. doi"; pages: " 
            10.3389/fnmol.2013.00009. Print 2013"
COMMENT     Sequence Label: pcDNA3-ClopHensor-H148G-V224L
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..125
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             join(132..134,135..845)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSGNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(174..195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(435..454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ggatggctag ctggagccac ccgcagttcg
      121 aaaaaggcgc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      301 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      541 tggggcacaa gctggagtac aactacaaca gcggcaacgt ctatatcatg gccgacaagc
      601 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      721 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      781 acatggtcct gctggagttc ctgaccgccg ccgggatcac tctcggcatg gacgagctgt
      841 acaagcgcgg atccgcgtct ggtggtggtg gtggtcta
//
