LOCUS       sequence-68537-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Mukhtarov M, Liguori L, Waseem T, Rocca F, Buldakova S, Arosio D, 
            Bregestovski P
  TITLE     Calibration and functional analysis of three genetically encoded 
            Cl(-)/pH sensors.
  JOURNAL   Front Mol Neurosci. 2013 Apr 18;6:9. doi: 10.3389/fnmol.2013.00009. 
            Print 2013.
  PUBMED    23616745
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-04-18"; volume: "6:9. doi"; pages: " 
            10.3389/fnmol.2013.00009. Print 2013"
COMMENT     Sequence Label: pcDNA3-ClopHensor-H148G-V224L
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             63..737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="DNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVV
                     EVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISHA
                     LKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHSG
                     SQ"
     primer_bind     458..477
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(772..789)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    778..889
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcgcggatc cgcgtctggt ggtggtggtg gtctagttcc acgtggatct gcctcaggag
       61 cagacaacac cgaggacgtc atcaaggagt tcatgcagtt caaggtgcgc atggagggct
      121 ccgtgaacgg ccactacttc gagatcgagg gcgagggcga gggcaagccc tacgagggca
      181 cccagaccgc caagctgcag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
      241 tgtcccccca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      301 actacatgaa gctgtccttc cccgagggct tcacctggga gcgctccatg aacttcgagg
      361 acggcggcgt ggtggaggtg cagcaggact cctccctgca ggacggcacc ttcatctaca
      421 aggtgaagtt caagggcgtg aacttccccg ccgacggccc cgtaatgcag aagaagactg
      481 ccggctggga gccctccacc gagaagctgt acccccagga cggcgtgctg aagggcgaga
      541 tctcccacgc cctgaagctg aaggacggcg gccactacac ctgcgacttc aagaccgtgt
      601 acaaggccaa gaagcccgtg cagctgcccg gcaaccacta cgtggactcc aagctggaca
      661 tcaccaacca caacgaggac tacaccgtgg tggagcagta cgagcacgcc gaggcccgcc
      721 actccggctc ccagtagtgc tctagagggc ccgtttaaac ccgctgatca gcctcgactg
      781 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      841 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
//
