LOCUS       sequence-68550-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human pSico-based U6 vector containing murine U6 promoter and sgRNA 
            targeting GAL4 UAS promoter (negative control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pU6-sgGAL4-4
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(295..328)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     332..352
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        436..511
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        636..939
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
       61 agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta
      121 ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg
      181 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
      241 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataact
      301 tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt
      361 aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt gttggaacga
      421 ctagttaggc gtgtagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      481 tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag
      541 gcggccgcgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
      601 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      661 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      721 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      781 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      841 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      901 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      961 atcaatgggc gtgg
//
