LOCUS       sequence-68559-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ragusa MJ, Stanley RE, Hurley JH
  TITLE     Architecture of the Atg17 complex as a scaffold for autophagosome 
            biogenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. 
            Epub 2012 Dec 6.
  PUBMED    23219485
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.11.028"; journalName: "Cell"; date: 
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1501-12"
COMMENT     Sequence Label: Atg17-Atg31-Atg29-pST39 (Auto 16)
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             485..507
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(535..552)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(535..551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             773..784
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tacatcgagc tcaatgtccg agtccactct gaatctatca ctgtcaacgc taagttcttc
       61 ctcttcatca ttagatttat catcaccatg tgatgataat gttctcattt ggttttcctc
      121 caagctgaga ctatttgtcc tatgtgacaa aagctcatat tggtcgctga ttagtgtgat
      181 atcctcaaga gatcccgatt catccaattg gacaattatg acattttcaa gttcatctcc
      241 gaccttatct attcctgctg tcaaagcagc atcacttgaa tctactagtt catcattgtt
      301 gtcctcaaat atgtacttaa tgttagtggg aaacattgca taatcacttc cgtccgtgct
      361 ctttgattca ttattgtatg ctggttgatc atcgttgctg ggacccttgt tatttttgat
      421 attctcttct aatctgtact tgacattttt atcataaaca gtaactgtaa cattcatatg
      481 tatatctcct tcttaaagtt aaacaaaatt attcccgggg aattcgggga attcactggc
      541 cgtcgtttta caggatcttg tacagggccc ggatccctaa ggattcttca cgttgtaatt
      601 taaagtgtac agggaagaaa aatcgtcaat tttaccgggc cagattgtct caggaagata
      661 agttccattt tccgctataa aattctgacg ttcttcctgg tcctgagcat ctaaattctg
      721 caactgcttt tcacaatctt tcaatatagt tttcattctg tttgccacat cctttctcct
      781 ctttgcttct agaactaagt taccgtagct ttcttcaaaa ttatcataaa attcacaaag
      841 ttccttagtt gtttgaatat cttgtgtaca ggaatttttg aagctatcaa tcagattgga
      901 aatatcttta aagattaata gatattccaa atttctattg aaatcgttta ttatttcaga
      961 aacttcgctg tgtagttctg tcttttcctt tatttttgcc tgcaaaaatt gacc
//
