LOCUS       sequence-68561-e        7698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP6 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP6-bio
FEATURES             Location/Qualifiers
     source          1..7698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3078..3133
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3132..3151)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5369..5387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5455..5559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5560..5628,5629..6420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5778..5797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6591..7179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7080..7099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7333..7350
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7467..7488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7503..7508,7509..7526,7527..7533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7541..7557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7546..7568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7565..7581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7565..7581
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcctaca tcaacgagaa caacttcatc cggaacgagc
     1381 tgatcaatga gaagaaccac aacctgagaa acggcgccat gtacaacaac gacaagatcc
     1441 tgagcaagaa cgaggtggac accaacatcg agagcaacga gaactccatc cacgagagcg
     1501 gccacaagat cgacggcgag gaagtgctga aggccaacgt ggacgacatc acctacaaga
     1561 aaaagaacgt ggatgacagc gagatcccct tcagcggcta cgacatccag gccacctacc
     1621 agttccctag caccagcggc ggcaacaacg tgatccccct gcccatcaag cagagcggcg
     1681 agaaccagta caccgtgacc agcatcagcg gcatccagaa gggcgccaac ggactgaccg
     1741 gcgccaccga gaatatcgcc caggtggtgc aggccaacag cgagacaaac aagaacccca
     1801 cctcccacag caacagcgcc accacctccc tgaacaacaa catcctgggc tgggagtttg
     1861 gagggggcgc tcctcagaat ggcgccgctg aggacaagaa aaccgagtat ctgctggaac
     1921 agatcaagat ccctagctgg gaccggaaca acatccccga cgagaacgaa caggtcatcg
     1981 aggaccccca ggaagataac aaggacgagg atgaggacga ggaaaccgaa accgagaacc
     2041 tggaaaccga ggacgacaac aacgaggaaa tcgaggaaaa cgaagaggac gacatcgacg
     2101 aagagagcgt ggaggaaaaa gaggaagagg aagaaaagaa agaagaagag gaaaagaaag
     2161 aggaaaaaaa agaagagaag aaacccgaca acgagatcac caacgaagtg aaagaggaac
     2221 agaagtacag cagccccagc gacatcaacg cccagaacct gatcagcaac aagaacaaga
     2281 agaacgacga gacaaagaaa accgccgaga acatcgtgaa aaccctcgtg ggcctgttca
     2341 acgagaagaa cgagatcgac agcaccatca acaacctggt gcaggaaatg atccacctgt
     2401 tctccaacaa cggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     2461 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     2521 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     2581 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     2641 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     2701 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     2761 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     2821 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     2881 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     2941 ccaagatcca ggttttatcc aaagggttga attccggatc actgcatcat attctggatg
     3001 cacagaaaat ggtgtggaat catcgttaag tctagacacg tgattaatta aggatccccc
     3061 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     3121 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     3181 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3241 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3301 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3361 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3421 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3481 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3541 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3601 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3661 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3721 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3781 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3841 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3901 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3961 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     4021 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     4081 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     4141 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     4201 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4261 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4321 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4381 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4441 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4501 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4561 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4621 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4681 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4741 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4801 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4861 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4921 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4981 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     5041 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     5101 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     5161 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     5221 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5281 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5341 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5401 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5461 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5521 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5581 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5641 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5701 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5761 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5821 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5881 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5941 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6001 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6061 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     6121 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6181 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6241 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6301 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6361 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6421 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6481 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7261 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7321 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7381 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7441 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7501 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7561 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7621 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7681 agaactggta ggtatgga
//
