LOCUS       sequence-68562-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human pSico-based U6 vector containing murine U6 promoter and sgRNA 
            targeting endogenous CD71 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pU6-sgCD71-2
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(295..328)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     332..352
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        435..510
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
       61 agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta
      121 ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg
      181 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
      241 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataact
      301 tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt
      361 aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt gttgggacgc
      421 gctagtgtga gtgcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      481 caacttgaaa aagtggcacc gagtcggtgc tttttttctc gagtactagg atccattagg
      541 cggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
      601 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      721 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      781 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      841 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatggg
//
