LOCUS       sequence-68564-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dominant-negative EHD2 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Stoeber M, Stoeck IK, Hanni C, Bleck CK, Balistreri G, Helenius A
  TITLE     Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane
            through association with actin.
  JOURNAL   EMBO J. 2012 May 16;31(10):2350-64. doi: 10.1038/emboj.2012.98. Epub
            2012 Apr 13.
  PUBMED    22505029
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.98"; journalName: "EMBO J"; date: "2012-05-16- 
            16"; volume: "31"; issue: "10"; pages: "2350-64"
COMMENT     Sequence Label: EHD2-T72A-mCherry
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..14)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccctgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catggtggcg
      121 accggtacca ctttgtacaa gaaagttggg cactcggcgg agcccttgtg gcgtcgcttg
      181 gagggtggca ccaggcgacg gggcaggttg gcgggcagcc cgtggccttc cagcttggcc
      241 tcgatgaggt ggctggccag cgcgaactct tcatcatcca gcatgccgtc gcggtccaca
      301 tcgctgagct tccagatgcg ccccagcact gagttgggga gcttggtccc caccatccag
      361 gtcttggcct tggagccgct cagcttgccg tcggcaggcg ccaggttgta gaagatctcg
      421 tcgtatttgg acttgtcctt ggtcaccacc cactcggcct cgtcgtccga gccctcctcg
      481 ccgtcctcca tggcctcgtc aggtccccgc tccacaaacg ggcccatgtg ggtgccctca
      541 aaagcgcccc cctgcacgcc cacctcggtg ctctccagct cctcctgccg cagcaggggc
      601 atgagcttgg cgatgtcgtg cgtcagcatc tcgtccagtg cctccagcag cttcggcttc
      661 agcgagtgaa acttggtgaa gtcatgcgcc atcagcagct cctgcatttt ctggcaatca
      721 ggaaagtccc caggggagat gtgatgttcc agctgaatct tcgcaaagat gacgggcagt
      781 ttga
//
