LOCUS       sequence-68567-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector containing SFFV promoter, dCas9 that is 
            fused to 2x NLS and tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pHR-SFFV-dCas9-BFP
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa
       61 ttctcacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccggcca ccatggacaa
      121 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
      181 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      241 caagaagaac ctgatcggcg ccctgctgtt cgacagcgga gaaacagccg aggccacccg
      301 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      361 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      421 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      481 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
      541 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
      601 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
      661 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
      721 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
      781 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggca acctgattgc
      841 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
      901 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
      961 cgaccagtac gccga
//
