LOCUS       sequence-68570-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target EGFP gene that is stably integrated into HEK293 genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pMLS-SV40-EGFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           66..159
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           173..285
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(318..338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        323..652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      503..638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(704..725)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgactaggga taacagggta
       61 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
      121 tgctgggatt acttcttcag gttaacccaa cagaaggctc gagcaaccag aattcaaggg
      181 gctactttag gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata
      241 catttttaca aagctgaatt aaaatggtat aaattaaatc acttttttca attcgccagc
      301 acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcat
      661 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      721 cgacgta
//
