LOCUS       sequence-68574-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52
            promoter, targeting endogenous TRE elements of pTET07 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pSNR52-sgTET
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..308
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     misc_RNA        329..403
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      408..427
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(456..474)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(495..511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(495..511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(508..530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    519..535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(543..549,550..567,568..573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    588..609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(726..743)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcac tcggaattct ctttgaaaag ataatgtatg
       61 attatgcttt cactcatatt tatacagaaa cttgatgttt tctttcgagt atatacaagg
      121 tgattacatg tacgtttgaa gtacaactct agattttgta gtgccctctt gggctagcgg
      181 taaaggtgcg cattttttca caccctacaa tgttctgttc aaaagatttt ggtcaaacgc
      241 tgtagaagtg aaagttggtg cgcatgtttc ggcgttcgaa acttctccgc agtgaaagat
      301 aaatgatcac ttttctctat cactgatagt tttagagcta gaaatagcaa gttaaaataa
      361 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtggtgcttt ttttgttttt
      421 tatgtctctg cagagttcgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
      481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt ttcgtattgg gcgctcttcc
      721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      901 cataggctcg gcccccctga cgagcatcac aaaaatcgac gctcaagtca
//
