LOCUS       sequence-68576-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pInducer10-mir-RUP-PheS
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             134..736
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     633..653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(811..831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
       61 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
      121 acacgataat accatggcca ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
      181 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
      241 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
      301 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
      361 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gctcgcgcat
      421 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
      481 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
      541 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgctgg
      601 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
      661 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
      721 caagcccggt gcctgattaa ttaagcgcgt ctggaacaat caacctctgg attacaaaat
      781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
      841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcatt
//
