LOCUS       sequence-68584-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA4.BDD FVIII.H3(M2238V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Peters lab - FVIII constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BDD FVIII H3
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcgactct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagtt aagcttcgcg acgtacggcc gccaccatgc
      121 aaatagagct ctccacctgc ttctttctgt gccttttgcg attctgcttt agtgccacca
      181 gaagatacta cctgggtgca gtggaactgt catgggacta tatgcaaagt gatctcggtg
      241 agctgcctgt ggacgcaaga tttcctccta gagtgccaaa atcttttcca ttcaacacct
      301 cagtcgtgta caaaaagact ctgtttgtag aattcacgga tcaccttttc aacatcgcta
      361 agccaaggcc accctggatg ggtctgctag gtcctaccat ccaggctgag gtttatgata
      421 cagtggtcat tacacttaag aacatggctt cccatcctgt cagtcttcat gctgttggtg
      481 tatcctactg gaaagcttct gagggagctg aatatgatga tcagaccagt caaagggaga
      541 aagaagatga taaagtcttc cctggtggaa gccatacata tgtctggcag gtcctgaaag
      601 agaatggtcc aatggcctct gacccactgt gccttaccta ctcatatctt tctcatgtgg
      661 acctggtaaa agacttgaat tcaggcctca ttggagccct actagtatgt agagaaggga
      721 gtctggccaa ggaaaagaca cagaccttgc acaaatttat actacttttt gctgtatttg
      781 atgaagggaa aagttggcac tcagaaacaa agaactcctt gatgcaggat aggaatgctg
      841 catctgctcg ggcctggcct aa
//
