LOCUS       sequence-68585-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA4.BDD FVIII.H3(M2238V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Peters lab - FVIII constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: BDD FVIII H3
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             541..570
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             586..603
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(626..643)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    632..743
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag
       61 agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc
      121 acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct
      181 caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacagt gaaagtcaca
      241 ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc
      301 atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag
      361 gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta
      421 ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg
      481 gaggttctgg gctgcgaggc acaggacctc tactgagcgg ccgctcgagg tcacccattc
      541 gaacaaaaac tcatctcaga agaggatctg aatatgcata ccggtcatca tcaccatcac
      601 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
      661 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      721 taataaaatg aggaaattgc atcgca
//
