LOCUS       sequence-68588-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Loewer S, Cabili MN, Guttman M, Loh YH, Thomas K, Park IH, Garber M,
            Curran M, Onder T, Agarwal S, Manos PD, Datta S, Lander ES, 
            Schlaeger TM, Daley GQ, Rinn JL
  TITLE     Large intergenic non-coding RNA-RoR modulates reprogramming of human
            induced pluripotent stem cells.
  JOURNAL   Nat Genet. 2010 Dec;42(12):1113-7. Epub 2010 Nov 7.
  PUBMED    21057500
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2010-12"; volume: "42(12)"; pages: "1113-7. Epub 
            2010 Nov 7"
COMMENT     Sequence Label: pLKO.1-lincRNA-RorR-sh2 (Linc-sh2)
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      9..144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(164..183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(165..183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(193..210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(193..209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(202..224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      351..731
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(438..457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     648..669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
       61 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      121 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
      181 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      241 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      301 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
      661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
      721 gccgatttcg gcctattggt taaaaaatga gctgatttaa ca
//
