LOCUS       sequence-68592-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Oliveira AF, Yasuda R
  TITLE     An improved Ras sensor for highly sensitive and quantitative 
            FRET-FLIM imaging.
  JOURNAL   PLoS One. 2013;8(1):e52874. doi: 10.1371/journal.pone.0052874. Epub 
            2013 Jan 14.
  PUBMED    23349692
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e52874. doi"; pages: " 
            10.1371/journal.pone.0052874. Epub 2013 Jan 14"
COMMENT     Sequence Label: FRas2-M Acceptor
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(239..262)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(437..456)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgtggagaa gtctgaacac tgcacagcac tctggttgca ggcccctcac cttgagtgct
       61 ttcataaggc agtcatgcaa gctcattcca tttcgcacat tgaccactgt tctttgctcg
      121 ttcggcaaga aaacacggat agtgttgctt gtcttagaag gatccatggt agatctgtag
      181 gcgccggtgg agtggcggcc ctcggcgcgc tcgtactgtt ccacgatggt gtagtcctcg
      241 ttgtgggagg tgatgtccag cttgatgtcg gtcttgtagg cgccgggcag ctgcacgggc
      301 ttcttggcca tgtaggtggt cttgacctcg gcgtcgtagt ggccgccgtc cttcagcttc
      361 agcctcatct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggtggag
      421 gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
      481 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
      541 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      601 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtactgg
      661 aactgagggg acaggatgtc ccaggcgaaa gg
//
