LOCUS       sequence-68602-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for the amplification of spiking anchors in BeSD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Diamante L, Gatti-Lafranconi P, Schaerli Y, Hollfelder F
  TITLE     In vitro affinity screening of protein and peptide binders by 
            megavalent bead surface display.
  JOURNAL   Protein Eng Des Sel. 2013 Oct;26(10):713-24. doi: 
            10.1093/protein/gzt039. Epub 2013 Aug 26.
  PUBMED    23980186
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt039"; journalName: "Protein Eng Des Sel"; date: 
            "2013-10"; volume: "26"; issue: "10"; pages: "713-24"
COMMENT     Sequence Label: pIVEX-anchor
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(222..240)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      236..283
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(649..654,655..671,672..677))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(698..714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(698..714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(711..733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(746..752,753..770,771..776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    791..812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
       61 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
      121 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatccggc
      181 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      241 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      301 atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
      361 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
      421 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
      481 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
      541 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
      601 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat
      661 cgatgataag ctgtcaaaca tgagaattcg taatcatgtc atagctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      841 agtcgggaaa cc
//
