LOCUS       sequence-68603-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SNAP-GFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Diamante L, Gatti-Lafranconi P, Schaerli Y, Hollfelder F
  TITLE     In vitro affinity screening of protein and peptide binders by 
            megavalent bead surface display.
  JOURNAL   Protein Eng Des Sel. 2013 Oct;26(10):713-24. doi: 
            10.1093/protein/gzt039. Epub 2013 Aug 26.
  PUBMED    23980186
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/protein/gzt039"; journalName: "Protein Eng Des Sel"; date: 
            "2013-10"; volume: "26"; issue: "10"; pages: "713-24"
COMMENT     Sequence Label: pIVEX-SNAP-GFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             622..644
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(675..694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(676..694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(778..797)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttac tcatgcggcc gcttcccaag
       61 cctggcttcc ccaaccggtg gccttcatgg gccagaagcc attccttcac ggccagtcca
      121 ccctcgtaac cgcccacatc caaatccccc tggaccactc tgtggcacgg gatcaggata
      181 gggacaggat tgccactcag tgccgtcttc actgctgccg tggctgcggg gttgcctgcc
      241 agggctgcta agtgagagta agaaatcact tctccgaatt tcacaacctt cagcagcttc
      301 cataacacct gtctggtgaa cgactcttgc tggaaaacgg gatggtgaag agccggcacg
      361 gggaactctt cgatagcctc gggctggtgg aaataggcat tcagccaggc tgtagcctgc
      421 atcaggggct ccggacctcc gagaaccgca gcgggggctg ggacctccac ggcatcagct
      481 gcagacgtcc ccttgcccag aaaaattatt tcgtgcagac cctgctcaca accagacagc
      541 tccagcttcc ccaaagggct gtccagtgtg gtgcgtttca tttcacaatc cttgtccatg
      601 gagcccaggc ccatatgtat atctccttct taaagttaaa caaaattatt tctagaggga
      661 aaccgttgtg gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
      721 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      781 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      841 gtttcggcgt gggtatggtg gcaggccccg tggccgg
//
