LOCUS       sequence-6861-ex        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA
  TITLE     Transcriptional repression and developmental functions of the 
            atypical vertebrate GATA protein TRPS1.
  JOURNAL   EMBO J. 2001 Apr 2. 20(7):1715-25.
  PUBMED    11285235
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2001 Apr 2. 20(7):1715-25."
COMMENT     Sequence Label: p060 pCS mTRPS1
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    4520..4654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4544..4563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4598..4617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4771..4791)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4771..4789)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4810..4826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4810..4826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4823..4845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4834..4850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4858..4864,4865..4882,4883..4888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4903..4924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5041..5058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5212..5800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5292..5311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5971..6762,6763..6831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6594..6613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6832..6936)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6962..7417)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7099..7120)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7311..7330
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7489..8473
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        8508..8526
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8508..8525
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aattcacgcg ggggcggaga gtgaggtcct gacaagcgat aacatttctg ataaagaccc
       61 gatctcattg caatctccag cgtcctcttt ttcgctgctg ctcggtggct ccggacctcg
      121 cgtcctcctg atcgctctct cgcctttgcc tatttttttt tttaaacaac aacaacaaca
      181 acaaaaaacc ccaacaacac cccctcccct cttcccaccc ggcaccgggc acatcctagc
      241 tctatttcct ttctctttct ctcgcgcccc caataatcca agatcaactc tgcaaacaac
      301 agaagacggt tcatggctct ggccgccgcg ccaccatctt tcgggctgcg gagggtgttc
      361 ctgacgatta atcaacagat atggtccgga aaaagcaccc ccctctgaga aacgttgcta
      421 gtgaaggcga gggtcaaacc ctggagccaa cagctacaga gagcaaggta tctgggaaga
      481 acaaagaatt gtctgcagat cagatgtcag aaaatacaga tcagagtgat gttgcagagt
      541 tgaactcgaa ggaggaacac agcacgcatg gccaagagcc atcttccagc ggtaagaagg
      601 acttgcaaat ctcaggcctg agtgagaagg ctggcttcaa ttatgaaagc cccagtaagg
      661 gagggagcct tgtctccttc ccacatgatg aggtaacaga cagaaatatg ttggctttct
      721 catctccagc tgctggggga gtctgcgagc ccttgaagtc tcctcaaaga gcagaggcag
      781 atgaccctca agatatggcc tgcaccccat caggggattc attggagaca aaggaagagc
      841 ataaaatgtc accaaaggcc actgaggaaa cagggccagt gcagagtggt caagccaatt
      901 gtcaaggttt gagtccagtt tccgtggcct caaaaaaccc acaagtgcct tcagatgggg
      961 gtgtcagact gagtaaaccc aaaggtgact tactggtgaa tgacaaccca gacccggcac
     1021 ctctgtctcc agagcttcag gactttaaat gcaatatctg tggatacggt tactatggca
     1081 atgaccccac agatctgatt aagcacttcc gaaagtatca cttaggactg cataatcgca
     1141 ccaggcagga tgctgagctg gacagcaaaa tcttggccct tcataacatg gtgcagttca
     1201 gccattccaa agacttccag aaggtcaacc gttctgtgct ttctggcgtg ctgcaggata
     1261 tcagctcttc acggcctgct ttactaaatg gaacctatga tgtacaggtc acttcaggag
     1321 gaactttcat tggcattgga cggaaaactc cagattgcca aggcaacacc aagtatttcc
     1381 gctgcaaatt ctgcaatttc acttatatgg gaaactcctc aactgaacta gagcaacatt
     1441 ttcttcagat gcacccgaat aaaatcaaag tgtctctccc gtcctccgag ggtgtgaaac
     1501 cttcagagaa aaactctaac aaatccatcc ctgcgcttcg agccagtgac tctggggatg
     1561 tgggaaagtg gcaggacaaa atgacagtca aagctggaga tgacacccct gtcggctact
     1621 cagtgcccat caagcccctc gattcctcac gacaaaatgg tacagaggcc accagttatt
     1681 actggtgtaa attttgtagt ttcagctgtg agtcatccag ctcccttaag ctgctagaac
     1741 attatggcaa gcagcacgga gcagtgcagt cgggcggcct taatccagag ttgaatgaca
     1801 agcttcccag gggctctgtc attaatcaga atgatctagc caaaagtgta gaaggagagc
     1861 cattgaccaa gccagaaaag ggcttgagtg gggctaaaaa gaaggacttc cccagcaagg
     1921 gagcagagga taatatggta acgagctata actgtcagtt ctgtgacttt agatattcca
     1981 aaagccatgg ccctgatgtg attgtagtgg gtccacttct tcgtcattat cagcaacttc
     2041 ataacattca caagtgtacc attaagcact gtccattctg tcccagaggc ctttgcagcc
     2101 cagaaaagca ccttggagag attacttatc cgtttgcttg tagaaaaagt aattgttccc
     2161 actgtgcact gttgctgttg cacttgtctc ctggggtggc cggaagttcg agagtcaaac
     2221 accaatgcca ccagtgttcc ttctctaccc ccgatgtaga tgtgctcctc tttcactatg
     2281 agaccgtgca tgagtcccaa gcatcggatg tcaaacagga ggccaaccac ctgctaggat
     2341 ctgacgggca gcaggctgtc agggacagca aggagcactc gtgcaccaaa tgcgatttta
     2401 taacccaggt ggaagaagag atctcgagac actacaggag agcacacagc tgctataaat
     2461 gtcgtcagtg cagtttcaca gctgctgaca ctcagtcact attggagcac ttcaacacag
     2521 ttcactgcca ggaacaggaa atcactactg ctaatggcga agagggtggc catgccatac
     2581 ccaccatcaa agaagagccc aaaattgacc tcaaggtcta cagtctgcta aacccagact
     2641 ctaaaatggg agaaacagtt cccgagagca tagtcaagag agagaaactt gatgacaagg
     2701 aagggctgaa agataaaatc tggactgaga gttcaactga tgaccttcgt ggtgtggctt
     2761 ggagaggggc agacatccta cggggtagtc catcctacac tcaagcaagc ttagggctct
     2821 tgacgcctgt gtccagctcc caagagcaga caaagactct gagggacagc cccaatgttg
     2881 aagctgccca cctggcacga cctatgtatg gcttggcagt agataccaag ggcttcctgc
     2941 aaggggctcc tgctggcagc gagaagtctg catctcttac ccagcagtat cctgcctccg
     3001 gagagagcaa gaccaaggat gaatcccagt cgctgttacg gaggcgaaga ggatctggtg
     3061 ttttttgtgc caattgcctg accacaaaga cctctctctg gcgaaagaat gcaaatggcg
     3121 gatatgtatg caatgcatgt ggtctctacc agaaacttca ctcgactccc aggcctttaa
     3181 acatcattaa acaaaacaac ggcgagcaga ttattagaag acggacaaga aagcgcctta
     3241 acccagaggc acttcaggct gagcagctca acaaacagca gaggggaagt ggggaggagc
     3301 aggtcaatgg aagcccctta gagaggaggt cagaagatca cttaagtgaa agtcatccga
     3361 gagaaattcc acttccgagc ctgagtaaat acgaagccca gggttcattg actaaaagcc
     3421 attctgctca gcagccagtc ctggtcagcc aagctctgga tatccacaaa aggatgcagc
     3481 ctttgcacat tcagataaaa agtcctcagg aaagtactgg agatccagga aatagttcat
     3541 ccgtatctga tgggaaagga agttctgaaa gaggcagccc catcgaaaag tacatgagac
     3601 ctgcaaaaca ccccaactat tctccaccag gcagccccat cgaaaagtac caatacccgc
     3661 tttttggagt tccttttgta cataatgact tccagagtga agctgattgg ctacggttct
     3721 ggagtaaata taagctctcc gttcctggga atccgcacta cttgagtcat gtgcctggct
     3781 taccaaatcc ttgccaaaac tatgtgcctt atcccacctt caatctgcct cctcatttct
     3841 cagctgttgg atcagacaat gacattcctc tagatttggc gatcaagcat tccagacctg
     3901 ggccaactgc aaatggtgcc tccaaggaga aaaccaaggc accaccaact gtaaaaaatg
     3961 aagatccctt gaatgtagtt aagacggaga aagttgacag aagcactcaa gatgaacttt
     4021 caacgaaatg tgtgcactgt ggcattgttt ttctggatga agtgatgtat gctttgcaca
     4081 tgagttgcca tggtgacagt ggacctttcc agtgcagcat atgccagcat ctttgcacgg
     4141 acaaatatga cttcacaaca catatccaga ggggcctgca taggaacaat gcacaagcag
     4201 aaaaaaatgg aaaacctaaa gagtaaaacc ttagcactta gcacaattaa atagaaatag
     4261 gttttcttga tgggaattca atagcttgta atgtcttatg aagacctatt aaaaaaataa
     4321 ttcatagagc ctgccttatc caacataaaa ttcctttttt tttttaattt ctttcttttg
     4381 atgagtaggt taccaagatt ttaaaatgaa acatatggtc agtgagaaag aatgaaatta
     4441 atagataatt aaaaaaaaaa aaaaaaagcg gccgcaaggc ctctcgagcc tctagaacta
     4501 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
     4561 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4621 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4681 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggcgcgcc gcggcgccaa
     4741 tgcattgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     4801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6961 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7021 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7081 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7141 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7201 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7261 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7321 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7381 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     7441 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagga ccatagccaa
     7501 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa
     7561 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa
     7621 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacctggc actgtgccaa
     7681 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca
     7741 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag
     7801 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc
     7861 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt
     7921 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc
     7981 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat
     8041 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg
     8101 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga
     8161 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat
     8221 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg
     8281 gaagtacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg
     8341 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg
     8401 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc
     8461 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac
     8521 tatagaatac aagctacttg ttctttttgc aggatcccat cgattcg
//
