LOCUS       sequence-68625-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ecDHFR based destable domain function at low temperature for 
            regulating protein stability in Caenorhabditis elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ
  TITLE     Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.
  JOURNAL   PLoS One. 2013 Aug 22;8(8):e72393. doi: 
            10.1371/journal.pone.0072393.
  PUBMED    23991108
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-22"; volume: "8(8):e72393. doi"; pages: " 
            10.1371/journal.pone.0072393"
COMMENT     Sequence Label: pBMN ltDHFR(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..508
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDHVIGMENAMPWSLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSADEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVGGDTHFPDYEPDDWGSVFSEFHDADAQNSHSYCFVILGRR"
     primer_bind     complement(557..578)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(818..837)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaccatcctc tagactgccg gatccgccac catgatcagt ctgattgcgg cgttagcggt
       61 agatcacgtt atcggcatgg aaaacgccat gccgtggagc ctgcctgccg atctcgcctg
      121 gtttaaacgc aacaccttaa ataaacccgt gattatgggc cgccatacct gggaatcaat
      181 cggtcgtccg ttgccaggac gcaaaaacat tatcctcagc agtcaaccga gtacggacga
      241 tcgcgtaacg tgggtgaagt cggcggatga agccatcgcg gcgtgtggtg acgtaccaga
      301 aatcatggtg attggcggcg gtcgcgttta tgagcagttc ttgccaaaag cgcaaaaact
      361 gtatctgacg catatcgacg cagaagtggg aggcgacacc catttcccgg attacgagcc
      421 ggatgactgg ggatcggtat tcagcgaatt ccacgatgct gatgcgcaga actctcacag
      481 ctattgcttt gtgattctgg ggcggcgagc atgcgtgagc aagggcgagg agctgttcac
      541 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      601 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      661 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
      721 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      781 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      841 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      901 cttcaaggag gacggcaaca tcctggggca caagctgga
//
