LOCUS       sequence-6863-ex        5497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA
  TITLE     Transcriptional repression and developmental functions of the 
            atypical vertebrate GATA protein TRPS1.
  JOURNAL   EMBO J. 2001 Apr 2. 20(7):1715-25.
  PUBMED    11285235
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2001 Apr 2. 20(7):1715-25."
COMMENT     Sequence Label: p062 pCS xTRPS1 deltaN
FEATURES             Location/Qualifiers
     source          1..5497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1544..1678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1568..1587)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1622..1641
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1795..1815)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1795..1813)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1834..1850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1834..1850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1847..1869)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1858..1874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1882..1888,1889..1906,1907..1912))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1927..1948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2065..2082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2236..2824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2316..2335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2995..3786,3787..3855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3618..3637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3856..3960)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3986..4441)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4123..4144)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4335..4354
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4513..5497
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcc ccaccatggt gtctgtaaac
      121 caagatcagc aaaaatcctc aagagacagc cccaatgtgg aagcagcaca tctagccagg
      181 ccagtgtatg gcttgtctat agaacccaag ggatttcaag gagtgacagc aggtgctagt
      241 ggagaaaagt ctgggcagca tacaccacag tatcctacag caggagatag caaatcaaaa
      301 gatgaatccc agtcactgct aaggaggcgt agaggatccg gtgtgttctg tgccaactgt
      361 ctgaccacaa agacatccct ctggaggaaa aatgccaacg gtggctatgt ctgcaatgcg
      421 tgtggccttt accagaaact tcactcgact cccagaccgt taaatataat taagcagaac
      481 aatggtgagc agatcattag gagacgaaca agaaagcgcc ttaacccaga agcactgcag
      541 cctgaacagt taactaaaca tcaaagagcg agtagtgaag aacaagctaa tggaagtcct
      601 ttagatataa ggtcagagga ccactcaatg gaaggtcatc aaagagaaaa tcagcagctt
      661 agtatgaata aatatgggtc tcaagcttca ttgactaaaa gccattctgc tcagcagacc
      721 atgattgtca gtcaaaccat ggatatacac aaacggatgc aacctttgca cattcagata
      781 aaaagccctc aggaaagcag tggagaacca ggaaacagct catctgtttc tgatggcaaa
      841 ggaagctcag aaagaggaag tccaattgag aaatacatga gacctataaa gcatcctaat
      901 tactctccac ctggaagccc aatagaaaaa tatcagtacc cactctttgg acttcctttt
      961 gttcacaatg acttccagag tgaagctgac tggctgagat tctggagtaa atataaactg
     1021 tcagttcctg gaaatccgca ctacttgagt catgtgcctg gcctgccaaa tccctgccca
     1081 aattatgtgc cttatcccac ctttaatctg cctgctcaat attcctctgt tgggtcagac
     1141 aatgacattc ctctagattt ggcaatgaag cattccagac ctggatcagg aacaaatgga
     1201 gactctaagg aaaaatcaaa gtcacccgtg agtgtaaagg atgatggtcc tttgaatgta
     1261 actaaaatag agaaatcaga taagagtacg caagatgaac tttcaacaaa atgtgtgcac
     1321 tgtggcattg tctttctgga tgaggttatg tatgctctac atatgagttg ccatggggaa
     1381 agtggacctt ttcagtgtag tatatgccaa catctttgca cggacaagta tgacttcaca
     1441 actcatattc agagaggcct acataggaac attgcacaag ctgaaaagaa tggaaaaaat
     1501 aaagatctcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag
     1561 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1621 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1681 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1741 attcgcggcg cgccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta
     1801 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1861 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1921 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1981 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2041 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2101 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2161 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2221 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2281 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2341 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2401 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2461 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2521 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2581 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2641 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     2701 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2761 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2821 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2881 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2941 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3001 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3061 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3121 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3181 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3241 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3301 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3361 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3421 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3481 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3541 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3601 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3661 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3721 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3781 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3841 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3901 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3961 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     4021 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4081 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4141 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4201 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4261 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     4321 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4381 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4441 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4501 ctattacgcc aggaccatag ccaattcaat atggcgtata tggactcatg ccaattcaat
     4561 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
     4621 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
     4681 atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
     4741 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
     4801 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
     4861 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
     4921 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
     4981 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
     5041 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
     5101 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
     5161 cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc
     5221 ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt
     5281 aacttggcaa gtacattact attggaagta cgccagggta cattggcagt actcccattg
     5341 acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg
     5401 gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta
     5461 atgggaggtc tatataagca atgctcgttt agggaac
//
