LOCUS       sequence-6864-ex        5732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA
  TITLE     Transcriptional repression and developmental functions of the 
            atypical vertebrate GATA protein TRPS1.
  JOURNAL   EMBO J. 2001 Apr 2. 20(7):1715-25.
  PUBMED    11285235
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2001 Apr 2. 20(7):1715-25."
COMMENT     Sequence Label: p072 pCS xGATA4 + xTRPS1(C119)
FEATURES             Location/Qualifiers
     source          1..5732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1779..1913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1803..1822)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1857..1876
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2030..2050)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2030..2048)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2069..2085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2069..2085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2082..2104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2093..2109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2117..2123,2124..2141,2142..2147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2162..2183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2300..2317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2471..3059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2551..2570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3230..4021,4022..4090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3853..3872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4091..4195)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4221..4676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4358..4379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4570..4589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4748..5732
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg agaagatgag ggatgtgacc
      121 ttctgatttg ctgattgtct ctagacttat ctcaggcctg gtttccatta gattggggat
      181 tcgacgtgag atatctgctt gctttctctt gaagaacata catgcacagc cgtatctaac
      241 gttgaagagc gctgagacta tcggatctgt ggattgagtt gccaggatca ggatgtatca
      301 gagtatagct atggccacta accatggtcc ctctggctat gaggggactg ggagcttcat
      361 gcacagtgct actgctgcta cctcacctgt ctatgtgccc accaccaggg tctcctccat
      421 gatccacagc ctaccttacc tccaaaccag cggctcatct caacaaggaa gcccagtttc
      481 tggccacaac atgtgggcac aagccggagt ggaatcttct gcctacaacc caggaacttc
      541 tcatccccca gtgtctccca gattcacttt ctcctccagc ccccctatca cagcaccctc
      601 cagcagagag gtctcctaca gtagccccct aggcatctca gctaatggga gagagcagta
      661 cagcaggggg ctgggtgcca cctatgcaag cccttaccca gcctatatga gtccagacat
      721 gggtgctgcc tggactgctt ctcccttcga cagctccatg ctccacaacc tccagaacag
      781 agcagtgacg tcaaggcacc caaacataga gttttttgac gatttttccg agggccgaga
      841 atgtgtcaac tgtggagcaa tgtcaacccc actttggagg cgggatggaa caggccacta
      901 tctatgcaat gcttgcggat tgtaccataa gatgaatggg atcaaccgtc ctctgatcaa
      961 gccccaaagg cgactgtctg cgtctcgccg ggtgggtcta tcctgtgcca actgtcatac
     1021 aaccactact acactctggc gtcgtaatgc tgagggggaa cctgtatgca atgcgtgtgg
     1081 cctttacatg aagctacacg gggtccctcg gccgctagca atgaaaaagg aagggatcca
     1141 gacacgaaag cgcaaaccca agaacctcag caagtctaaa acactaacag gccaaagtgg
     1201 cagtgacagc ctcactcctt ccaccagctc cacaaactcc atgggagagg aaatgcgtcc
     1261 aataaagatt gagccaggac tgtcccctcc atatgaccac tcaaattcaa tatctcaggc
     1321 atctgcatta tctacaatca caagccatgg atcatcatat tacccaatgc caagcttttc
     1381 ctctagattt ggcaatgaag cattccagac ctggatcagg aacaaatgga gactctaagg
     1441 aaaaatcaaa gtcacccgtg agtgtaaagg atgatggtcc tttgaatgta actaaaatag
     1501 agaaatcaga taagagtacg caagatgaac tttcaacaaa atgtgtgcac tgtggcattg
     1561 tctttctgga tgaggttatg tatgctctac atatgagttg ccatggggaa agtggacctt
     1621 ttcagtgtag tatatgccaa catctttgca cggacaagta tgacttcaca actcatattc
     1681 agagaggcct acataggaac attgcacaag ctgaaaagaa tggaaaaaat aaagattaaa
     1741 actcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca
     1801 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1861 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1921 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg
     1981 cggcgcgccg cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag
     2041 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2881 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4201 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     4261 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     4321 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     4381 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     4441 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4501 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4561 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4621 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     4681 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     4741 acgccaggac catagccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt
     4801 ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt
     4861 ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc
     4921 ggacctggca ctgtgccaac tggggagggg tctacttggc acggtgccaa gtttgaggag
     4981 gggtcttggc cctgtgccaa gtccgccata ttgaattggc atggtgccaa taatggcggc
     5041 catattggct atatgccagg atcaatatat aggcaatatc caatatggcc ctatgccaat
     5101 atggctattg gccaggttca atactatgta ttggccctat gccatatagt attccatata
     5161 tgggttttcc tattgacgta gatagcccct cccaatgggc ggtcccatat accatatatg
     5221 gggcttccta ataccgccca tagccactcc cccattgacg tcaatggtct ctatatatgg
     5281 tctttcctat tgacgtcata tgggcggtcc tattgacgta tatggcgcct cccccattga
     5341 cgtcaattac ggtaaatggc ccgcctggct caatgcccat tgacgtcaat aggaccaccc
     5401 accattgacg tcaatgggat ggctcattgc ccattcatat ccgttctcac gccccctatt
     5461 gacgtcaatg acggtaaatg gcccacttgg cagtacatca atatctatta atagtaactt
     5521 ggcaagtaca ttactattgg aagtacgcca gggtacattg gcagtactcc cattgacgtc
     5581 aatggcggta aatggcccgc gatggctgcc aagtacatcc ccattgacgt caatggggag
     5641 gggcaatgac gcaaatgggc gttccattga cgtaaatggg cggtaggcgt gcctaatggg
     5701 aggtctatat aagcaatgct cgtttaggga ac
//
