LOCUS       sequence-6865-ex        5494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Malik TH, Shoichet SA, Latham P, Kroll TG, Peters LL, Shivdasani RA
  TITLE     Transcriptional repression and developmental functions of the 
            atypical vertebrate GATA protein TRPS1.
  JOURNAL   EMBO J. 2001 Apr 2. 20(7):1715-25.
  PUBMED    11285235
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2001 Apr 2. 20(7):1715-25."
COMMENT     Sequence Label: p073 pCS xGATA4tr + xTRPS1(C119)
FEATURES             Location/Qualifiers
     source          1..5494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1541..1675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1565..1584)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1619..1638
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1792..1812)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1792..1810)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1831..1847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1831..1847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1844..1866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1855..1871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1879..1885,1886..1903,1904..1909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1924..1945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2062..2079)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2233..2821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2313..2332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2992..3783,3784..3852))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3615..3634
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3853..3957)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3983..4438)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4120..4141)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4332..4351
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4510..5494
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg agaagatgag ggatgtgacc
      121 ttctgatttg ctgattgtct ctagacttat ctcaggcctg gtttccatta gattggggat
      181 tcgacgtgag atatctgctt gctttctctt gaagaacata catgcacagc cgtatctaac
      241 gttgaagagc gctgagacta tcggatctgt ggattgagtt gccaggatca ggatgtatca
      301 gagtatagct atggccacta accatggtcc ctctggctat gaggggactg ggagcttcat
      361 gcacagtgct actgctgcta cctcacctgt ctatgtgccc accaccaggg tctcctccat
      421 gatccacagc ctaccttacc tccaaaccag cggctcatct caacaaggaa gcccagtttc
      481 tggccacaac atgtgggcac aagccggagt ggaatcttct gcctacaacc caggaacttc
      541 tcatccccca gtgtctccca gattcacttt ctcctccagc ccccctatca cagcaccctc
      601 cagcagagag gtctcctaca gtagccccct aggcatctca gctaatggga gagagcagta
      661 cagcaggggg ctgggtgcca cctatgcaag cccttaccca gcctatatga gtccagacat
      721 gggtgctgcc tggactgctt ctcccttcga cagctccatg ctccacaacc tccagaacag
      781 agcagtgacg tcaaggcacc caaacataga gttttttgac gatttttccg agggccgaga
      841 atgtgtcaac tgtggagcaa tgtcaacccc actttggagg cgggatggaa caggccacta
      901 tctatgcaat gcttgcggat tgtaccataa gatgaatggg atcaaccgtc ctctgatcaa
      961 gccccaaagg cgactgtctg cgtctcgccg ggtgggtcta tcctgtgcca actgtcatac
     1021 aaccactact acactctggc gtcgtaatgc tgagggggaa cctgtatgca atgcgtgtgg
     1081 cctttacatg aagctacacg gggtccctcg gccgctagca atgaaaaagg aagggatcct
     1141 tcctctagat ttggcaatga agcattccag acctggatca ggaacaaatg gagactctaa
     1201 ggaaaaatca aagtcacccg tgagtgtaaa ggatgatggt cctttgaatg taactaaaat
     1261 agagaaatca gataagagta cgcaagatga actttcaaca aaatgtgtgc actgtggcat
     1321 tgtctttctg gatgaggtta tgtatgctct acatatgagt tgccatgggg aaagtggacc
     1381 ttttcagtgt agtatatgcc aacatctttg cacggacaag tatgacttca caactcatat
     1441 tcagagaggc ctacatagga acattgcaca agctgaaaag aatggaaaaa ataaagatta
     1501 aaactcgagc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     1561 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1621 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1681 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     1741 cgcggcgcgc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
     1801 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3961 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     4021 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4081 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4141 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4201 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4261 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4321 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4381 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     4441 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4501 ttacgccagg accatagcca attcaatatg gcgtatatgg actcatgcca attcaatatg
     4561 gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca attcaatatg
     4621 gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca attcaatatg
     4681 gcggacctgg cactgtgcca actggggagg ggtctacttg gcacggtgcc aagtttgagg
     4741 aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc aataatggcg
     4801 gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg ccctatgcca
     4861 atatggctat tggccaggtt caatactatg tattggccct atgccatata gtattccata
     4921 tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat ataccatata
     4981 tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt ctctatatat
     5041 ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc ctcccccatt
     5101 gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca ataggaccac
     5161 ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc acgcccccta
     5221 ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat taatagtaac
     5281 ttggcaagta cattactatt ggaagtacgc cagggtacat tggcagtact cccattgacg
     5341 tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac gtcaatgggg
     5401 aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg
     5461 ggaggtctat ataagcaatg ctcgtttagg gaac
//
