LOCUS       sequence-68707-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3545
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     327..346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        327..345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      301 tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac cggcctcgag
      361 aagctcgcgt tatcggtcct ttaattggtg gacaaattta cattagtctc ggacattctg
      421 ctcccgccct tatgggattt atcttcctta ttctcgcttt gattgttcta tcgaaagcga
      481 aatcaaacga ataatttata aataaaaaaa ccacctctaa aaggtgattt ttatttataa
      541 attacaattt ttcagcaata tcagtaattg ctttatcaac tgctgctttt tggctatcaa
      601 tcaaagcaac acgtgctgta atttgtttaa ttgccatttc aattgaacgt ttcaagcctt
      661 ggttttctaa ttttggttca aagaaagctt gagctctcta gaactagtgg taccgcatgc
      721 ctgcagtacc catggtgagt gcctccttat aatttatttt gtagttcctt cgaacgaaat
      781 cattgtatct aacaaacttc agaatttaat cagagccgtt tattatgctc gcgttatcga
      841 caataatatt attaccaata ctttctcaag atagaattaa gactgtttta gatttgttaa
      901 tgtttctatt g
//
