LOCUS       sequence-68710-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express cGFP transcript ending in a poly(A) tail [using the SV40 
            poly(A) signal].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Wilusz JE, JnBaptiste CK, Lu LY, Kuhn CD, Joshua-Tor L, Sharp PA
  TITLE     A triple helix stabilizes the 3' ends of long noncoding RNAs that 
            lack poly(A) tails.
  JOURNAL   Genes Dev. 2012 Nov 1;26(21):2392-407. doi: 10.1101/gad.204438.112. 
            Epub 2012 Oct 16.
  PUBMED    23073843
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.204438.112"; journalName: "Genes Dev"; date: 
            "2012-11-1- 1"; volume: "26"; issue: "21"; pages: "2392-407"
COMMENT     Sequence Label: pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    407..528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(444..463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     498..517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc gagctgaccg
       61 gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac aactacaacg
      121 cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg aacttcaaga
      181 tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag cagaataccc
      241 ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc cagagcgccc
      301 tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc gtgaccgccg
      361 ccgccatcac ccacggcatg gatgagctgt acaagtgagc ggccgcaact tgtttattgc
      421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttagc ggccgcgctg
      541 tgcggtaggc attgaggcag ccagcgcagg ggcttctgct gagggggcag gcggagcttg
      601 aggaaaccgc agataagttt ttttctcttt gaaagataga gattaataca actacttaaa
      661 aaatatagtc aataggttac taagatattg cttagcgtta agtttttaac gtaattttaa
      721 tagcttaaga ttttaagaga aaatatgaag acttagaaga gtagcatgag gaaggaaaag
      781 ataaaaggtt tctaaaacat gacggaggtt gagatgaagc ttcttcatgg agtaaaaaat
      841 gtatttaaaa gaaaattgag agaaaggact acagagcccc ga
//
