LOCUS       sequence-68717-e        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3535VA
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     327..346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        327..345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      301 tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac cggcctcgag
      361 tctagaagcg ctgtcgacga gctcgcatgc gaattcctgc agcccgggac tagtggtacc
      421 ggatccatgc agagtctcct gttttacaac cgggtgtaca tagcgaaata cttgtaatgc
      481 gtggtgatgc acctgaatct ttcttcgaaa cagataccaa atccaagcta aaatcttttg
      541 tactcatttt gagtgcctcc ttataattta ttttgtagtt ccttcgaacg aaatcattgt
      601 atctaacaaa cttcagaatt taatcagagc cgtttattat gctcgcgtta tcgacaataa
      661 tattattacc aatactttct caagatagaa ttaagactgt tttagatttg ttaatgtttc
      721 tattgtcagt atagttataa gactagatct gatccgtagc ggttttcaaa atttgcaacc
      781 aggaatgaat tactgtgaaa caactaaccg agaaaaaatg gactggttaa gaaaacagta
      841 ccaagaagag ctgaaaaaac tagaatcgtc taaaaagccc ctagaagacg atttaagcca
      901 tttagaagag ttgcttgatg aaaagaccaa ggaatatatt aaaatcgata aacccagcga
      961 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
     1021 agaattactc tatgaagcgc c
//
