LOCUS       sequence-68722-e        2235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses MepTrac-H194E in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-meptrac-H194E
FEATURES             Location/Qualifiers
     source          1..2235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             676..927
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     864..885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(991..1012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1252..1271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtcttaca attttacagg tacgcctaca ggcgaaggaa cgggtggtaa ctcgttgaca
       61 acagatttga atacacaatt tgacttggcc aacatgggat ggatcggtgt ggcttcagca
      121 ggtgtgtgga ttatggtccc aggtatcggt ttattatatt ctggtttatc caggaaaaag
      181 catgctttat ccttgctttg ggcctcgatg atggcttccg ccgtgtgtat tttccaatgg
      241 tttttctggg gatactcatt agctttctca cacaacacta gaggtaacgg ttttattggt
      301 accttggaat tctttgggtt tcgtaacgtt ttaggagccc catctagtgt cagttctctt
      361 cccgatatac tgtttgccgt ttaccaaggt atgtttgccg cagtcaccgg tgccctaatg
      421 ctaggtggtg cctgcgagag ggcaaggttg tttcctatga tggtgttctt gtttttatgg
      481 atgactattg tttattgtcc tattgcatgc tgggtctgga atgccgaggg ttggttggtc
      541 aaattgggta gcttggacta tgcaggtggt ttatgtgtcg agttaacatc tggacatggt
      601 ggtctagttt acgctttgat actgggtaag cgtaatgacc ctgttacacg tttgtctaac
      661 gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
      721 aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc
      781 gacggccccg tgctgctgcc cgacaaccac tacctgagcg tccagtccaa gctgagcaaa
      841 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      901 actctcggca tggacgagct gtacaagggt ggtaccggtg gatctatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1021 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1141 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1201 aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa ggacgacggc
     1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1381 tttaataaag ggatgcccaa gtacaaacca cattccgtca cctcggtggt tttaggcaca
     1441 gtgttcttat ggtttggttg gatgttcttt aacggaggct ctgcaggtaa tgcaactata
     1501 cgagcatggt actctattat gtccacaaac ttagctgctg cttgcggtgg cttgacatgg
     1561 atggttatcg attatttcag atgcggaaga aagtggacta cagttggttt gtgttcaggt
     1621 atcatcgctg gcctagtggg tatcacccca gccgccgggt tcgtgccaat ctggtcagcc
     1681 gttgtcattg gtgtggttac tggtgcagga tgtaaccttg ctgttgactt aaagagtcta
     1741 ttgcgcatcg atgatggtct agattgttac tctatccatg gtgtgggtgg ttgtattggt
     1801 tctgtattaa ctggtatctt tgctgcagac tatgtaaatg ccactgcagg ctcttacatt
     1861 agtccaattg atggtggctg gatcaatcat cactataaac aagttggtta tcaattagca
     1921 ggtatatgcg ctgcactagc ctggactgtt actgtcacat ctatcttgct tctaactatg
     1981 aatgccattc catttttaaa actaagatta agtgctgatg aggaagaatt aggtaccgac
     2041 gctgctcaaa ttggtgaatt tacatacgag gaatccactg cttacatccc agaaccaatc
     2101 agatctaaaa catcggcaca aatgccacct cctcatgaaa acattgatga taagattgtg
     2161 ggtaacacag acgcagaaaa gaattctacg ccttccgacg cttcttctac taagaacact
     2221 gaccatatag tataa
//
