LOCUS       sequence-68723-e        9988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  General cloning vector for use in E. coli or D. radiodurans with 
            lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Meima R, Lidstrom ME
  TITLE     Characterization of the minimal replicon of a cryptic Deinococcus 
            radiodurans SARK plasmid and development of versatile Escherichia 
            coli-D. radiodurans shuttle vectors.
  JOURNAL   Appl Environ Microbiol. 2000 Sep;66(9):3856-67.
  PUBMED    10966401
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2000-09"; volume: "66(9)"; pages: 
            "3856-67"
COMMENT     Sequence Label: pRADZ1
FEATURES             Location/Qualifiers
     source          1..9988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9144..9803
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9210..9229)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattggagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagctc gcgaggcctc gagatctata gggaaaaggt ggtgaactac tgtggaagtt
     2221 actgacgtaa gattacgggt cgaccgggaa aaccctggcg ttacccaact taatcgcctt
     2281 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2341 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     2401 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     2461 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
     2521 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     2581 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     2641 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     2701 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     2761 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     2821 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     2881 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     2941 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     3001 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     3061 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     3121 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     3181 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     3241 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     3301 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     3361 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
     3421 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     3481 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     3541 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
     3601 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
     3661 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     3721 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     3781 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     3841 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
     3901 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
     3961 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
     4021 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
     4081 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     4141 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     4201 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     4261 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     4321 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
     4381 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
     4441 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
     4501 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     4561 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
     4621 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     4681 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
     4741 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     4801 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
     4861 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     4921 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     4981 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     5041 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     5101 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     5161 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaatta
     5221 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
     5281 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttcaagcta
     5341 attccggtgg aagccgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca
     5401 tctagaagct tctgcagacg cgtcgacgtc atatggatcc gattcgacct gcaggcatgc
     5461 aagctgtacg cctcagctac cattcttgca gccgcgttca gggtcttgac gtgttctttg
     5521 tatctatcag caaactgaga acccaattct ggatcatcct tgtaacgcca actctccgac
     5581 cattccgaag gaacgccttg tagtcctcct gagccgttag ggtatgtctt tagtccaatc
     5641 aaatgggaca tttgcagaca ggaacgcaca agtgtctcac gtcgcttctc tatttcaata
     5701 tctaaaaatt cttcactctt atcgttgctt gacatattaa aactgtctaa ctgatccagt
     5761 tcacttatat ggaaagtatc actaaagtca tggcttctca gaaattcaat acttcccatt
     5821 gatggggacc ggttaaccaa atcgataaaa atttttctgt catgtttcag ataagttttt
     5881 ggcgcgttga ttttataata aagccgccat tcttgtgcca acaacgccac gaaaagagcc
     5941 acagcagcag cggccacatc ccattgaaaa tctttcgcca gaccaaccgc caggagaacc
     6001 aagccgaaat agttagccca gcgaactaaa aaactatcta taaacgtatt aagaaacgtt
     6061 cgggtcatat ttccgtgaat catatcagga cgtaaatttg cacatgacgc atttccgggg
     6121 gcttttatcc ctcggacttc ttttcaggcc ccttctcggc ctttctgtga ccctgttttt
     6181 tcaggccgcg aactgcgcct ggtcgggcct gacccccaca cgctggcctt cgtactcacg
     6241 cagcctgccc agcaggtcgg ccagcgcggc attgaccacg gcggcgaggt tgcggggcgg
     6301 cgttccgcct gcggtctggt cgtgcttcac gtcgcgcagc acgcgcacca gcaccgcgcc
     6361 cacgtcgtcg gacacgtccc ggccagcgtc ggcggcgcgg gtgatgttcc aaatgagttt
     6421 ccgccagaag cccagcgagt cggccccgtc cccgaaagct gccgctagcg cccgcgcctg
     6481 cctgtccacg atctcggcgc ggtaggccgg gcgggtttcc cggtgggcgt cggcaagctc
     6541 ccacacgacg ttttcagcga gggagggcgc gggccggaca gtcatgttat cggaggggga
     6601 gggaagagcg gattttatcg cccaggtgct cacaacttcg tatgtcacgg caaccggggc
     6661 gcttttttcg cgcagttccc cgcctgtcac atcctcacag ggttcttgac tctccggcac
     6721 gctcttaggg agagggtgga gcatgttgta gacggtgcgc ccggccttcg cgtcggcgtt
     6781 caggtcgcgc cagttccggc cccaatcgtc atgcatcagg cggacatatc ccgccttccc
     6841 ttccagcacg cgcctcggct tgagcgtcac ggcccacagc gtcccggttg ccacagactc
     6901 gccgcgcagg tcgcccatgt gggcatcaca ggccaccagc ccgacccggc gcagatattg
     6961 aaggttctcg tagaaggcgg acttcttcag acccacatgc accatgagca gctcggcggg
     7021 caggtggaac acagcgcggg tgacgtgttc cgcatagccg caggcgcggg ccacgtccag
     7081 cgccaccgag aacagggcgc ggaagatgcg ccgggccgac tcgcggcagg gggcgtcatc
     7141 taggcaggcg gtgagggtct gcaccagttc ggcgcgtgtg gcgatggtca acggcgcggg
     7201 cgcgggctgg gactgctggg cgatggtggg cacgggcggc ggcgagatgg accgggccga
     7261 gctggaaatt tcccgtgtga gcgcaggttt aggcgcatgg ttaccttcct gcagtgaaat
     7321 ctgcgaaatc aggcccgctt cgaggagtcg gaccatgaat ggattgtgtc tcaagaaaaa
     7381 agcctcccct cttgtcagag gggagggtcc cggtctacca tgctctcagc gacgagatag
     7441 ctggggtttg tgaccctgca ctctgacaaa gccctcaccg aaaggtgggg gtttagtcat
     7501 ttggcctagt aaagcagaac agaggcgatt tgtcacgcaa aggcccgccg cccgagggga
     7561 aacaggcggc gggccaatgg ctcggcgttg ttagctgggg gtcgcaccag ggaagcacag
     7621 aagcccttcc ctgagcaggg tttcaccgtg tggtgtccct tcgtccttga aggcgaactg
     7681 gtaggccagg ccgtaatgct cctgcaccat cttttgctca tccaccgcga caaagaaacg
     7741 gcgggtgttc gccgggctgc tgagggtctg ccactcgccg gacgcgccca ccgcataggc
     7801 gtctccggct ccacattcct cggcctgctg atactgatac ttcgtccaca gcatcaccgc
     7861 cttatcgggc acctgcgcga agctcaccag cgccccgaac cgcccggcgc tcgtggtgaa
     7921 ggtggacgac tgcaccatgt cggacagttc cagcgtggca ggcgcgggcg tgttcgtcgg
     7981 cgcgtacacc gtccccgcct cgcacacgac cagcggggcg gcccccgtgc cgaactcggc
     8041 ggcgctgccg tttgccagcg tgagcgccga agacgccacc gcgcaggtac ccatgggtgc
     8101 cgaccaccag caggtccacg ccgtgcgccg cctcggtgag cacctcgatt ggacggcccc
     8161 tcagggtctg aaccctcccc tgcacgcccg cctccccgat gagtgtctgc gccgtctgct
     8221 gcgcctgccg ggcattctgc tccagcatct cccggacgta gtcgggattc agtcccgccc
     8281 actgaacgaa gccgctgacc ggcggctcct cgatcacagt cacgacctcc agctcggcgc
     8341 ctgccgcctt cccgagcgtt atggcctgct tcaaggcgcg ctggctgggc tcacttccgt
     8401 caaaggccac cacgaatcgc atccgtcctc ctccctgacc agtcaggttc agcaagcagg
     8461 gtaagcctct cccagcactc cggggcttca cacggccttg ggtcttttct gcatacccat
     8521 gagcctcagt acccgacagt ttccggcagg gcttgaaaaa gttcggctga cacgcgagaa
     8581 ctggacgcgt ctcccgatgt cctggccaag ttgacccgct gcttcaccgc gcacttcccg
     8641 gagttccgca agaactaggt cgagctgctc tccctcatgg tcctcgccct ccttaggggc
     8701 aaggacgtcc ggcatgctaa actcgccgcg cgcttccccg gaagcgcgca caccgcctcc
     8761 gtcatccggc gggtggaacg cttcttcgac cgtcatcctc ttcggccagc tgatgtcgcc
     8821 cgggtcgttc tgacgctcct tcccgccgcg cagccacgcg aatttatcct tgaccggacc
     8881 aactggaagt atgggcagac ggacgtgaac gtcttgttgc tggccgtcat ttggcgggac
     8941 gtcgccatcc ccctgctcta cgagttgctg ccccatgggg gcagcagcga caccgagatt
     9001 cggcacaccc tgatggacga tgccctgtgc ctgctgtccg ccgctgacat ccgggtgctg
     9061 tattagccga ccgcgaattc ccagcttgag taggacaaat ccgccgagct tcgacgagat
     9121 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     9181 tatcccaatc gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     9241 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     9301 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     9361 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     9421 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     9481 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     9541 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     9601 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     9661 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     9721 atgccgtctg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     9781 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
     9841 gtgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcgt cgaggcggca
     9901 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     9961 gaatgcatcc cgcaagaggc ccggcagt
//
