LOCUS       sequence-68724-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   LeBlanc DJ, Lee LN, Abu-Al-Jaibat A
  TITLE     Molecular, genetic, and functional analysis of the basic replicon of
            pVA380-1, a plasmid of oral streptococcal origin.
  JOURNAL   Plasmid. 1992 Sep;28(2):130-45.
  PUBMED    1409970
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "1992-09"; volume: "28(2)"; pages: "130-45"
COMMENT     Sequence Label: pDL278
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..74
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(75..92)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(75..91)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(84..106)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      565..651
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      743..770
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
       61 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ccgggaatta
      241 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
      301 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
      361 tcggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      421 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      481 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      541 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      601 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
      661 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      721 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
      781 caaactcttc ctgtcgtcat atctacaagc catcccccca cagatacggt aaactagcct
      841 cgtttttgca tcaggaaagc agctgttttg gcggatgaga gaagattttc agcctgatac
      901 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
      961 cggtggtccc acctgacc
//
