LOCUS       sequence-68728-e        2280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses AmTrac1;2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-AmTrac1;2
FEATURES             Location/Qualifiers
     source          1..2280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             751..1002
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     939..960
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1066..1087)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1327..1346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggacaccg caaccaccac atgctctgcc gtagatctat ctgccctcct atcctcttct
       61 tctaactcaa catcttccct cgccgcggca acctttttat gttcccaaat ttcaaacatc
      121 tccaacaaac tctccgacac aacttatgcc gtcgacaaca cgtatctcct cttctccgcc
      181 taccttgtct ttgccatgca gctcggtttc gctatgcttt gtgctggatc agtccgagcc
      241 aagaacacta tgaacatcat gcttaccaat gtccttgatg ctgccgctgg agccatctct
      301 tactacctct tcggattcgc attcgccttt ggtacacctt ccaacggatt catcggtcgc
      361 caccatagct tcttcgcttt aagctcttac cctgaacgcc ccggctccga cttcagcttt
      421 ttcctctacc aatgggcttt tgccatagcc gcggccggaa tcactagcgg ttccatcgcc
      481 gagcgaacgc aattcgttgc ttaccttatc tactctactt tcttgaccgg ttttgtttac
      541 ccgacagtct cgcactggtt ctggtcaagt gatggatggg ctagcgcgtc ccggtctgac
      601 aacaatctct tgtttggctc aggtgctatt gatttcgcag gttcaggagt tgttcacatg
      661 gtaggtggaa ttgccggttt atgtggagcg ttagttgaag gaccaagaat aggtagattt
      721 gaccggttgt ccaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
      781 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
      841 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtccag
      901 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      961 accgccgccg ggatcactct cggcatggac gagctgtaca agggtggtac cggtggatct
     1021 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1081 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1141 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1201 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1261 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     1321 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1381 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1441 aagctggagt acaactttaa ttcaggccgg tccgtggctt tacgtggtca cagtgcatcc
     1501 cttgtcgtgc ttggtacctt cttgttgtgg tttggatggt atgggtttaa ccctggttcc
     1561 tttttaacca ttcttaaagg ctacgacaag tctcggccat attatggtca atggagcgct
     1621 gtaggtcgca ccgcggtcac cacaacgctt tctggctgca ccgctgcgtt gactactcta
     1681 ttcagtaaac ggcttttagc aggtcattgg aacgttattg acgtatgcaa cggacttcta
     1741 ggcggctttg cagctataac ctccggatgt gccgtggtgg agccgtgggc tgctatagta
     1801 tgtggctttg tggcatcatg ggttttaatc ggatttaact tgcttgccaa gaaacttaaa
     1861 tatgatgacc cactcgaggc tgctcagctc cacggtggat gtggagcatg gggattaatc
     1921 tttaccgggc tgttcgcaag gaaagaatac gttaacgaga tttactccgg tgataggcct
     1981 tacggactgt tcatgggcgg gggaggaaaa ctgctcgccg cgcagatcgt tcagattatt
     2041 gtgatcgttg ggtgggtgac ggtaactatg ggaccgttgt tttatgggtt acataagatg
     2101 aatcttttga ggatatcagc agaagatgag atggcaggaa tggacatgac acgtcatgga
     2161 ggatttgctt acgcatacaa tgacgaagac gacgtgtcga ctaaaccatg gggtcatttc
     2221 gctggaagag tggagcctac aagccggagc tcgactccta caccgacctt gactgtttga
//
