LOCUS       sequence-68729-e        2229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses AmTrac-IS in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   De Michele R, Ast C, Loque D, Ho CH, Andrade SL, Lanquar V, 
            Grossmann G, Gehne S, Kumke MU, Frommer WB
  TITLE     Fluorescent sensors reporting the activity of ammonium transceptors 
            in live cells.
  JOURNAL   eLife. 2013 Jul 2;2:e00800.
  PUBMED    23840931
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2013-07-2"; volume: "2"; pages: "e00800"
COMMENT     Sequence Label: pDR-AmTrac-IS
FEATURES             Location/Qualifiers
     source          1..2229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             724..975
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     912..933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1039..1060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1300..1319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtcaggag caataacatg ctctgcggcc gatctcgcca ccctacttgg ccccaacgcc
       61 acggcggcgg ccgactacat ttgcggccaa ttaggcaccg ttaacaacaa gttcaccgat
      121 gcagccttcg ccatagacaa cacctacctc ctcttctctg cctaccttgt cttcgccatg
      181 cagctcggct tcgctatgct ttgtgctggt tctgttagag ccaagaatac gatgaacatc
      241 atgcttacca atgtccttga cgctgcagcc ggaggactct tctactatct ctttggttac
      301 gcctttgcct ttggaggatc ctccgaaggg ttcattggaa gacacaactt tgctcttaga
      361 gactttccga ctcccacagc tgattactct ttcttcctct accaatgggc gttcgcaatc
      421 gcggccgctg gaatcacaag tggttcgatc gcagagagga ctcagttcgt ggcttacttg
      481 atatactctt ctttcttaac cggatttgtt tacccggttg tctctcactg gttttggtcc
      541 ccggatggat gggccagtcc ctttcgttca gcggatgatc gtttgtttag caccggagcc
      601 attgactttg ctggctccgg tgttgttcac atggttggtg gcatagcagg tttatggggt
      661 gctcttattg aaggtcctcg tcgtggtcgg ttcgagaaaa tctctaacgt ctatatcaag
      721 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac
      781 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg
      841 ctgctgcccg acaaccacta cctgagcgtc cagtccaagc tgagcaaaga ccccaacgag
      901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      961 gacgagctgt acaagggtgg taccggtgga tctatggtga gcaagggcga ggagctgttc
     1021 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1081 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1141 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1201 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1261 cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1321 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1381 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt taatggtggt
     1441 cgcgctattg ctctgcgcgg ccactctgcc tcgctagtag tcttaggaac cttcctccta
     1501 tggtttggat ggtatggttt caaccccggt tccttcacta agatactcgt tccgtataat
     1561 tctggttcca actacggcca atggagcgga atcggccgta cagcggttaa caccacactc
     1621 tcaggatgca cagcagctct aaccacactc tttggtaaac gtctcctatc aggccactgg
     1681 aacgtaacgg acgtttgcaa cgggttactc ggtgggtttg cggccataac cgcaggttgc
     1741 tccgtcgtag agccatgggc agcgattgtg tgcggcttca tggcttctgt cgtccttatc
     1801 ggatgcaaca agctcgcgga gcttgtacaa tatgatgatc cactcgaggc agcccaacta
     1861 catggagggt gtggcgcgtg ggggttgata ttcgtaggat tgtttgccaa agagaagtat
     1921 ctaaacgagg tttatggcgc caccccggga aggccatatg gactatttat gggcggagga
     1981 gggaagctgt tgggagcaca attggttcaa atacttgtga ttgtaggatg ggttagtgcc
     2041 acaatgggaa cactcttctt catcctcaaa aggctcaatc tgcttaggat ctcggagcag
     2101 catgaaatgc aagggatgga tatgacacgt cacggtggct ttgcttatat ctaccatgat
     2161 aatgatgatg agtctcatag agtggatcct ggatctcctt tccctcgatc agctactcct
     2221 cctcgcgtt
//
