LOCUS       sequence-68731-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral vector encoding a fusion between codon-optimized 
            P.aeruginosa exoY and myc-tagged destabilized FKBP12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Ochoa CD, Alexeyev M, Pastukh V, Balczon R, Stevens T
  TITLE     Pseudomonas aeruginosa exotoxin Y is a promiscuous cyclase that 
            increases endothelial tau phosphorylation and permeability.
  JOURNAL   J Biol Chem. 2012 Jul 20;287(30):25407-18. doi: 
            10.1074/jbc.M111.301440. Epub 2012 May 25.
  PUBMED    22637478
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.301440"; journalName: "J Biol Chem"; date: 
            "2012-07-20- 20"; volume: "287"; issue: "30"; pages: "25407-18"
COMMENT     Sequence Label: pMA3200
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..334)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLGDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQGAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     ELE"
     CDS             complement(335..364)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 attcctgcat ttattccagt tccagaagct ccacatcgaa gacgagagtg gcatgtggtg
       61 ggatgatgcc tgggtgccca gtggcaccat aggcataatc tggagatata gtcagtttgg
      121 ctccctgacc cacactcatc tgggcaaccc cttcttccca gcctcggatc acctcctgct
      181 tgcctagcat aaacttaaag ggcttgtttc tgtcccgaga ggaatcgact ttctttccat
      241 ctccaagcat cccggtgtag tgcaccacac aggtctggcc gcgcttgggg aaggtgcgcc
      301 cgtctcctgg ggagatggtt tccacctgca ctcccaggtc ctcctcgctg atgagtttct
      361 gctccccaac cagctccacc ttcctctgga agaagtccag ggcctcctgg aagccgggcc
      421 tcaggggcac gttccagtcg gggtgggcgg tgaagtggta gccgttgccc ttgatgcact
      481 ccaccagcag gctgaagctc ttcctgtcgg ccaccacgca cacctcgtcg aacctcacgc
      541 tctcctcgcc caccctgtgc tccatcgccc tgggcaggta gaaggtggcg gggaagttgt
      601 cgcccatgtg gctgccgggg ttgccggcgt cgtcgctgtg gtggaacatc tccctgtgct
      661 cgcccctgcc caggcagtcg ttcagggcgt ccaccagctg cctggtcctg ggggtgatgt
      721 tgcccctggc catgtcctcc ctgc
//
