LOCUS       sequence-68742-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana 
            from the Arabidopsis U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
  TITLE     Targeted mutagenesis in the model plant Nicotiana benthamiana using 
            Cas9 RNA-guided endonuclease.
  JOURNAL   Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
  PUBMED    23929340
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08-8- 8"; volume: "31"; 
            issue: "8"; pages: "691-3"
COMMENT     Sequence Label: pICH86966::AtU6p::sgRNA_PDS
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      113..365
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        392..466
                     /label=AtU6 promoter
                     /note="consensus promoter from the three Arabidopsis U6 
                     snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 
                     2013)"
     misc_RNA        487..562
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(574..598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    663..687
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
       61 tatcgccttc ttgacgagtt cttctgagcg ggtctggatc tagagtcaag cagatcgttc
      121 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
      181 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
      241 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
      301 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
      361 agatcgacgc tgcaagaatt caagcttgga gtgatcaaaa gtcccacatc gatcaggtga
      421 tatatagcag cttagtttat ataatgatag agtcgacata gcgattgccg ttaatttgag
      481 agtccagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      541 aaaagtggca ccgagtcggt gctttttttc tagacccagc tttcttgtac aaagttggca
      601 ttacgctact agaggatgca catgtgaccg agggacacga agtggtttaa actatcagtg
      661 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
      721 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc caaccacagg
      781 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      841 cacaaaa
//
