LOCUS       sequence-68745-e        7399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a 
            T7 promoter for in vitro transcription, germline-compatible 5'UTR, 
            tbb-2 3'UTR and a polyA sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Katic I, Grosshans H
  TITLE     Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979578
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pIK86
FEATURES             Location/Qualifiers
     source          1..7399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     439..455
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        474..492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             539..4642
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             539..4642
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4658..4678
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4685..4705
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5178..5194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5178..5194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5191..5213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5202..5218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5226..5232,5233..5250,5251..5256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5271..5292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5409..5426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5580..6168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5660..5679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6339..7130,7131..7199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6962..6981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7200..7304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7372..7390
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggttgt aaaacgacgg ccagtgaatt cgagctcggt acctaatacg
      481 actcactata ggaatacacg gaattctaga tgatccccgc gtaccgagct cagaaaaaat
      541 ggataaaaag tacagtattg gactggatat tggaacaaat tcggtgggat gggccgtgat
      601 tacagatgag tataaggtgc caagtaagaa gttcaaggtt cttggaaaca ctgatcgtca
      661 ttcaattaaa aagaacttga tcggagctct tttgttcgat tctggagaaa ctgcagaggc
      721 cacccgtctt aagagaaccg caagacgacg ttacacaaga cgaaagaaca gaatctgtta
      781 cttgcaagaa atcttttcaa atgagatggc taaagtcgat gatagtttct ttcatcgatt
      841 ggaagagtcc ttcctcgttg aagaggataa aaagcatgaa cgtcacccaa tttttggaaa
      901 tatcgtggat gaagtcgctt accatgagaa atatccaaca atttaccacc tgcgtaaaaa
      961 gcttgttgat tctactgata aggcagatct cagactgatt taccttgctt tggcacatat
     1021 gatcaaattc cgaggacact ttctgattga aggagatctt aatccagata actcagatgt
     1081 cgataaattg ttcattcaac tcgttcagac ttacaaccaa cttttcgaag agaacccaat
     1141 caacgcctcg ggagttgatg ccaaggctat tctgagtgct agactttcga aaagtcgtag
     1201 actcgaaaat ctgattgcac agttgccagg agagaaaaag aatggattgt ttggaaacct
     1261 cattgccctc tccctgggac ttaccccaaa cttcaagtca aacttcgatc ttgctgaaga
     1321 tgcaaagttg caactctcta aggatacata cgatgatgat ttggataatc tcctggcaca
     1381 gattggagat caatatgccg atctgttcct tgctgcaaaa aacctttcgg atgctattct
     1441 tttgagtgat atcttgcgag ttaatacaga aatcactaag gctccacttt ccgcatcaat
     1501 gatcaagaga tacgatgaac atcaccaaga tttgacactc ctgaaagcat tggttcgaca
     1561 acagctccca gaaaagtaca aggagatttt ctttgatcag tcgaagaacg gatacgctgg
     1621 atacatcgat ggaggagcaa gtcaagaaga gttctacaag ttcatcaagc caatcctcga
     1681 aaagatggat ggaactgaag agcttttggt taagcttaac cgtgaagatc tcctgcgaaa
     1741 acagcgtacc tttgataatg gatcaattcc acatcaaatc caccttggag aattgcatgc
     1801 tattctccga cgtcaagagg atttctatcc atttcttaag gataacagag aaaagatcga
     1861 gaagatcttg actttccgaa ttccatatta cgttggacca ctcgcccgtg gaaactctag
     1921 atttgcttgg atgacaagaa aatcggaaga gactattacc ccatggaatt tcgaagaggt
     1981 tgtggataaa ggagcatctg cccaatcgtt catcgaacga atgactaact tcgataagaa
     2041 cctgccaaac gaaaaggttc ttccaaagca tagtcttttg tacgaatact tcactgtgta
     2101 caatgagctt accaaggtta agtatgtgac agaaggaatg cgaaaaccag ctttcttgtc
     2161 aggagagcag aaaaaggcaa ttgttgatct cctgtttaag acaaatcgta aagtgactgt
     2221 caagcaactt aaggaagatt acttcaaaaa gatcgagtgc tttgattcgg ttgaaattag
     2281 tggagtggag gatagattta atgcatcttt gggaacatac catgatcttt tgaagatcat
     2341 caaagataag gatttcttgg ataatgaaga gaacgaagat attctcgagg atatcgtttt
     2401 gacactcact ctgtttgaag atcgtgagat gattgaagag agattgaaaa catacgctca
     2461 cctcttcgat gataaagtga tgaagcaact taaaagacga cgttatactg gatggggaag
     2521 actgtcacga aaacttatta atggaatccg tgataagcag agtggaaaaa ctattctcga
     2581 ttttctgaaa tccgatggat tcgcaaatag aaactttatg caactgatcc atgatgattc
     2641 tcttacattc aaggaagata tccaaaaagc ccaggtttct ggacagggag attcgctgca
     2701 tgaacacatt gccaatcttg ctggatcgcc agcaattaaa aagggaatcc tgcaaacagt
     2761 taaagtcgtt gatgaacttg ttaaggtgat gggacgacac aaaccagaaa atattgtgat
     2821 cgaaatggct cgtgagaacc aaacaactca gaagggacaa aagaattcta gagaacgaat
     2881 gaagcgaatt gaagagggaa tcaaagaact gggatcgcaa attcttaagg aacatccagt
     2941 cgagaatact caattgcaga acgaaaagct ttacttgtac tacctccaga acggaagaga
     3001 tatgtacgtt gatcaagaat tggatatcaa tcgactcagt gattatgatg tcgatcacat
     3061 tgttccacaa tccttcctta aggatgattc aatcgataac aaggtgttga cacgatccga
     3121 taagaatcgt ggaaaaagtg ataacgtccc atccgaagag gtggtcaaaa agatgaagaa
     3181 ctactggaga cagctcctga acgctaagtt gatcactcag cgtaagttcg ataatctcac
     3241 caaggcagaa agaggaggac ttagtgagtt ggataaggcc ggattcatta aacgacagtt
     3301 ggtggaaaca cgtcaaatta ctaaacatgt cgcacaaatc cttgattcac gtatgaacac
     3361 caagtacgat gaaaacgata agttgattag agaggtcaaa gttatcacat tgaaatccaa
     3421 gctcgtgtca gatttccgaa aggatttcca attctacaag gtccgtgaaa tcaacaacta
     3481 ccatcacgca cacgatgcct atttgaatgc cgttgtggga actgctttga ttaaaaagta
     3541 tccaaagctc gaatctgagt tcgtttacgg agattacaag gtctacgatg ttagaaagat
     3601 gatcgctaag tccgaacaag agatcggaaa ggctacagca aagtacttct tttattcaaa
     3661 catcatgaac ttctttaaga ccgaaattac actcgctaac ggagagattc gtaaaagacc
     3721 acttatcgaa accaatggag aaacaggaga gattgtctgg gataagggaa gagattttgc
     3781 aactgtgcga aaagtccttt ctatgccaca ggttaacatt gtgaaaaaga ctgaagtgca
     3841 aaccggagga ttcagtaaag agtccatcct gccaaagcgt aactcggata agcttatcgc
     3901 tagaaaaaag gattgggatc caaaaaagta cggaggattt gattctccaa ctgtggcata
     3961 ttcggtcttg gtcgttgcca aagtcgaaaa gggaaagtca aaaaagctta agtctgttaa
     4021 ggaacttttg ggaatcacaa tcatggaaag atcatctttc gagaaaaatc caatcgattt
     4081 ccttgaagca aagggataca aggaggttaa aaaggatctg atcatcaagc ttccaaagta
     4141 ctctctcttc gaactggaga atggacgaaa acgtatgttg gcctcggctg gagaactcca
     4201 aaagggaaac gagctcgctc tgccaagtaa atatgtgaat ttcctttact tggcatcaca
     4261 ttacgaaaag cttaagggat ctccagaaga taatgagcaa aagcagctgt ttgtcgaaca
     4321 gcataaacac tacctcgatg aaattatcga gcaaatctca gaattctcta agagagttat
     4381 cttggcagat gccaacttgg ataaagtgct ctcagcctac aacaagcata gagataagcc
     4441 aatccgagaa caagctgaga acattatcca ccttttcacc ttgacaaatc tcggagctcc
     4501 agccgctttc aagtacttcg ataccacaat cgatagaaag cgatatactt ccaccaaaga
     4561 agttctggat gcaacactta ttcatcaatc aatcactgga ctgtatgaaa ctcgtattga
     4621 tttgtcgcaa ctcggaggag atggaagtgg atcgatgcca aagaagaagc gtaaggtcga
     4681 agatccaaaa aaaaaaagaa aagttgatta gatgcaaaat cctttcaagc attcccttct
     4741 tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt
     4801 aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa
     4861 tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa ttccattttg
     4921 aagtgcttta aacccgaaag gttgagaaaa atgcgagcaa aaaaaaaaaa aaaaaaaaaa
     4981 aaaaaaaaaa aaaaaaaaaa aaaaagcgct agcgcttttt tttttttttt tttttttttt
     5041 tttttttttt tttttttttt ttgctcaaat atttgtattg tgttcgttga gtgacccaac
     5101 aaaaagagga aactttattg tgccgccaag aaaaaagtct cagcgagtga tattattgac
     5161 acgctggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7381 atcacgaggc cctttcgtc
//
