LOCUS       sequence-68747-e        7605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP7 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP7-bio
FEATURES             Location/Qualifiers
     source          1..7605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2985..3040
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3039..3058)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5276..5294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5362..5466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5467..5535,5536..6327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5685..5704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6498..7086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6987..7006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7240..7257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7374..7395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7410..7415,7416..7433,7434..7440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7448..7464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7453..7475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7472..7488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7472..7488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaccc ccgtgaacaa cgaagaggac caggaagaac
     1381 tgtacatcaa gaacaagaag ctggaaaagc tgaagaacat cgtgtccggc gacttcgtgg
     1441 gcaactacaa gaacaacgag gaactgctga acaagaagat cgaagaactg cagaactcca
     1501 aagaaaagaa cgtgcacgtg ctgatcaacg gcaacagcat catcgacgag atcgagaaga
     1561 acgaggaaaa cgacgataac gaagagaaca acgacgacga caacacctac gagctggaca
     1621 tgaacgacga cacctttctg ggccagaaca acgatgccca cttcgagaac gtggacgacg
     1681 acgccgtgga gaacgagcag gaagatgaga acaaagagaa gtccgagagc ttccccctgt
     1741 tccagaacct gggcctgttc ggcaagaacg tgctgtccaa agtgaaggcc cagagcgaga
     1801 cagacaccca gagcaagaat gagcaggaaa tcagcaccca gggccaggaa gtgcagaaac
     1861 cagcccaggg cggcgagagc accttccaga aggacctgga taagaagctg tacaacctgg
     1921 gcgacgtgtt caaccacgtg gtggacatca gcaacaagaa gaacaagatc aacctggacg
     1981 agtacggcaa gaagtacacc gacttcaaga aagaatacga ggacttcgtg ctcaactcta
     2041 aagagtacga catcatcaag aacctgatca tcatgttcgg ccaggaagat aacaaggcca
     2101 agaacggcaa gaccgatatc gtgtccgagg ccaagcacat gaccgagatc ttcatcaagc
     2161 tgttcaagga caaagagtac cacgagcagt tcaagaacta catctacggc gtgtacagct
     2221 acgccaagca gaacagccac ctgagcgaga agaagatcaa gcccgaggaa gagtacaaga
     2281 agttcctgga atacagcttc aatctgctga acaccatggg cgcgccgtcg acctccatca
     2341 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2401 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2461 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2521 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2581 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2641 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2701 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2761 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     2821 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     2881 ccggatcact gcatcatatt ctggatgcac agaaaatggt gtggaatcat cgttaagtct
     2941 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     3001 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3061 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     3121 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     3181 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     3241 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     3301 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     3361 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     3421 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     3481 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     3541 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     3601 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     3661 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     3721 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     3781 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     3841 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     3901 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     3961 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     4021 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     4081 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     4141 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     4201 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     4261 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     4321 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     4381 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     4441 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     4501 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     4561 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     4621 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     4681 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     4741 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     4801 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4861 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     4921 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     4981 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     5041 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5101 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5161 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     5221 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     5281 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5341 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5401 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5461 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5521 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5581 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5641 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5701 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5761 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5821 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5881 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5941 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6001 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6061 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6121 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6181 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6241 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6301 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6361 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6421 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6481 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6541 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6601 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6661 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6721 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6781 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6841 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6901 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6961 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7021 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7081 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7141 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7201 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7261 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7321 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7381 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7441 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7501 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     7561 caacgttgtt gccattgctg caggcgcaga actggtaggt atgga
//
