LOCUS       sequence-68749-e        8184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length AMA1 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: AMA1-bio
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3564..3619
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3618..3637)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5855..5873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5941..6045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6046..6114,6115..6906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6264..6283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7077..7665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7566..7585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7819..7836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7953..7974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7989..7994,7995..8012,8013..8019)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8027..8043
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8032..8054
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8051..8067
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8051..8067
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccaga attactggga gcacccttac cagaacagcg
     1381 acgtgtaccg gcccatcaac gagcacagag agcaccccaa agagtacgag taccccctgc
     1441 accaggaaca cacctaccag caggaagata gcggcgagga cgagaacacc ctgcagcacg
     1501 cctaccccat cgatcacgag ggcgccgaac cagcccccca ggaacagaac ctgttcagca
     1561 gcatcgagat cgtggagcgg agcaactaca tgggcaaccc ctggaccgag tacatggcca
     1621 agtacgacat cgaggaagtg cacggcagcg gcatcagagt ggacctgggc gaggatgccg
     1681 aagtggctgg aacccagtac agactgccca gcggcaagtg ccctgtgttc ggcaagggca
     1741 tcatcatcga gaacagcaac accgcctttc tgacccccgt ggccaccggc aaccagtacc
     1801 tgaaggacgg cggcttcgcc tttcctccca ccgagcccct gatgagcccc atgaccctgg
     1861 acgagatgcg gcacttctac aaggacaata agtacgtgaa gaacctggac gagctgaccc
     1921 tgtgttctcg gcacgccggc aacatgatcc ccgacaacga caagaactcc aactacaagt
     1981 accccgccgt gtacgacgac aaggataaga agtgccacat cctgtacatt gccgcccagg
     2041 aaaacaacgg ccccagatac tgcaacaagg acgagagcaa gcggaacagc atgttctgct
     2101 tcagacccgc caaggacatc agcttccaga actacgccta cctgagcaag aacgtggtgg
     2161 acaactggga gaaagtgtgc ccccggaaga atctgcagaa cgccaagttc ggcctgtggg
     2221 tggacggcaa ctgcgaggac atcccccacg tgaacgagtt ccccgccatc gacctgttcg
     2281 agtgcaacaa gctggtgttc gagctgtccg ccagcgacca gcccaagcag tacgagcagc
     2341 acctgaccga ctacgagaag atcaaagagg gcttcaagaa caagaacgcc gccatgatca
     2401 agagcgcctt tctgcccacc ggcgccttca aggccgacag atacaagagc cacggcaagg
     2461 gctacaactg gggcaactac aacaccgaga cacagaagtg cgagatcttc aacgtgaagc
     2521 ccacctgtct gatcaacaac gccgcctaca ttgccaccac cgccctgagc caccccatcg
     2581 aggtggagaa caacttcccc tgcagcctgt acaaggatga gatcatgaaa gagatcgagc
     2641 gggagagcaa gagaatcaag ctgaacgaca acgacgacga gggcaacaag aagatcattg
     2701 cccccagaat cttcatcagc gacgataagg acagcctgaa gtgcccctgc gaccccgaga
     2761 tggtgtccaa cagcgcctgc cggttcttcg tgtgcaaatg cgtggagaga agggccgaag
     2821 tgaccagcaa caacgaggtg gtggtcaaag aggaatacaa ggacgaatac gccgacatcc
     2881 ccgagcacaa gcccaccggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2941 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     3001 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     3061 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     3121 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     3181 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     3241 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     3301 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     3361 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     3421 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     3481 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     3541 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3601 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3661 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3721 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3781 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3841 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3901 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3961 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     4021 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     4081 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     4141 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     4201 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4261 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     4321 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     4381 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4441 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4501 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4561 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4621 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4681 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4741 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4801 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4861 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4921 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4981 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     5041 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     5101 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     5161 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     5221 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     5281 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5341 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5401 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5461 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5521 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5581 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5641 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5701 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5761 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5821 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5881 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5941 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6001 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6061 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6121 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6181 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6241 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6301 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6361 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6421 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6481 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6541 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6601 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6661 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6721 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6781 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6841 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6901 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6961 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7021 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7741 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7801 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7921 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7981 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8041 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     8101 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     8161 aggcgcagaa ctggtaggta tgga
//
