LOCUS       sequence-68750-e        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length EBA140 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: EBA140-bio
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5343..5398
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5397..5416)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7634..7652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7720..7824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7825..7893,7894..8685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8043..8062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8856..9444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9345..9364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9598..9615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9732..9753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9768..9773,9774..9791,9792..9798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9806..9822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9811..9833
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9830..9846
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9830..9846
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccatca tcggccggac cctgtacaac cggcaggacg
     1381 agagcagcga catcagcaga gtgaacagcc ccgagctgaa caacaaccac aagaccaaca
     1441 tctacgacag cgactacgag gacgtgaaca acaagctgat caacagcttc gtggagaaca
     1501 aggccgtgaa gaagaagcgg agcctgagct tcatcaacaa caaggccaag agctacgaca
     1561 tcatcccccc cagctacagc taccggaacg acaagttcaa cagcctgagc gagaacgagg
     1621 acaacagcgg caacaccaac agcaacaact tcgccaacac cgccgagatc agcatcggca
     1681 aggacaacaa gcagtacacc ttcatccaga agcggaccca cctgttcgcc tgcggcatca
     1741 agcggaagtc catcaagtgg atctgccggg agaacagcga gaagatcacc gtgtgcgtgc
     1801 ccgaccggaa gatccagctg tgtatcgcca acttcctgaa tagcagactg gaaaccatgg
     1861 aaaagttcaa agagatcttc ctgatcagcg tgaacaccga ggccaagctg ctgtacaaca
     1921 agaacgaggg caaggacccc agcatcttct gcaacgagct gcggaacagc ttcagcgact
     1981 tccggaactc cttcatcggc gacgacatgg acttcggcgg caataccgac agagtgaagg
     2041 gctacatcaa caagaagttc agcgactact acaaagaaaa gaacgtggag aagctcaaca
     2101 acatcaagaa agagtggtgg gagaagaata aggccaacct gtggaaccac atgatcgtga
     2161 accacaaggg caatatcgct aaagagtgtg ctatcatccc tgctgaggaa ccccagatca
     2221 atctgtggat caaagagtgg aacgagaact tcctgatgga aaagaagcgg ctgttcctga
     2281 acatcaagga taagtgcgtg gagaataaga agtacgaggc ctgtttcgga ggatgcagac
     2341 tgccctgcag cagctacacc agcttcatga agaaaagcaa gacccagatg gaagtgctga
     2401 ccaacctgta caagaaaaag aacagcggcg tggacaagaa caactttctg aacgacctgt
     2461 tcaagaagaa caataagaac gatctggatg atttcttcaa gaacgagaaa gaatacgacg
     2521 acctgtgcga ctgccggtac accgccacca tcatcaagag ctttctgaat ggccctgcca
     2581 agaacgacgt ggatatcgcc agccagatca acgtgaacga tctgcggggc ttcggctgca
     2641 actacaagag caacaacgag aagtcctgga actgcgccgg caccttcacc aacaagttcc
     2701 ccggcacatg cgagcctccc agacggcaga ccctgtgcct gggccggacc tatctgctgc
     2761 accggggcca cgaagaggac tacaaagagc atctgctggg cgccagcatc tacgaagccc
     2821 agctgctgaa gtacaagtac aaagagaagg acgagaacgc cctgtgcagc atcatccaga
     2881 acagctacgc cgacctggcc gacatcatca agggcagcga tatcatcaag gactactacg
     2941 gcaagaagat ggaagagaac ctgaacaaag tgaacaagga caagaagcgg aacgaggaaa
     3001 gcctgaagat cttccgcgag aagtggtggg acgagaacaa agaaaacgtg tggaaagtga
     3061 tgagcgccgt gctgaagaac aaagagacat gcaaggacta cgacaagttt cagaagatcc
     3121 cccagttcct gcggtggttt aaagagtggg gcgacgactt ctgcgagaag cgcaaagaga
     3181 agatctacag cttcgagagc ttcaaggtgg agtgcaagaa gaaggactgc gacgagaaca
     3241 catgcaagaa caagtgcagc gagtacaaga agtggatcga cctgaagaag tccgagtacg
     3301 agaaacaggt ggacaagtac accaaggata agaacaagaa gatgtacgac aacatcgacg
     3361 aagtgaaaaa caaagaggcc aacgtctacc tgaaagaaaa gtctaaagag tgcaaggacg
     3421 tcaacttcga cgacaagatc ttcaacgagg cccccaacga gtacgaggac atgtgcaaga
     3481 agtgcgacga gatcaagtac ctgaatgaga tcaaataccc caagaccaag cacgacatct
     3541 acgatatcga caccttctcc gacaccttcg gcgacggcac ccccatcagc atcaacgcca
     3601 acatcaacga gcagcagagc ggcaaggata ccagcaacac tggcaactct gaaacaagcg
     3661 acagtcctgt gtcccacgag cctgagagcg acgccgccat caacgtggaa aagctgtccg
     3721 gcgacgagtc cagcagcgag acacggggca tcctggacat caacgacccc agcgtgacca
     3781 acaacgtgaa tgaggtgcac gacgcctcca atacccaggg ctccgtgagc aataccgccg
     3841 acatcaccaa cggccacagc gagagcagcc tgaacagagc caccaacgcc caggacatca
     3901 agatcggcag atccggcaac gagcagagcg acaaccagga aaactccgcc cacagcagcg
     3961 acaatagcgg cagcctgacc atcggccagg tgcccagcga ggacaatacc cagaacacct
     4021 acgactccca gaacccccac agagacaccc ctaacgccct ggccagcctg cccagcgacg
     4081 ataagatcaa cgagatcgag ggcttcgaca gcagcagaga cagcgagaac ggcagaggcg
     4141 acaccacctc caacacccac gacgtgcggc ggaccaacat cgtgtccgag cggagagtga
     4201 actcccacga cttcatccgg aacggcatgg ccaacaacaa cgcccaccac cagtacatca
     4261 cccagatcga gaacaacggc atcatcagag gccaggaaga gtccgccggc aactccgtga
     4321 actacaagga caaccccaag cggagcaact tcgcctccga gaacgaccac aagaagaata
     4381 tccaggaata caacagccgg gacaccaaga gagtgcggga ggaaatcatc aagctgtcca
     4441 agcagaacaa gtgtaacaac gagtacagca tggaatactg cacctacagc gacgagagaa
     4501 acagcgcccc tggcccctgc tctcgcgaag aacggaagaa gctgtgctgc cagatctccg
     4561 actactgcct gaagtacttc aacttctaca gcatcgagta ctacaactgc atcaagagcg
     4621 agatcaagag ccccgagtac aagtgcttca agtccgaggg ccagagcagc atccccggcg
     4681 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     4741 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     4801 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     4861 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     4921 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     4981 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     5041 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     5101 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     5161 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     5221 tatccaaagg gttgaattcc ggatcactgc atcatattct ggatgcacag aaaatggtgt
     5281 ggaatcatcg ttaagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     5341 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     5401 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     5461 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     5521 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     5581 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     5641 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     5701 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     5761 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     5821 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     5881 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     5941 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     6001 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     6061 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     6121 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     6181 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     6241 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     6301 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     6361 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     6421 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     6481 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     6541 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     6601 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     6661 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     6721 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     6781 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     6841 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     6901 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     6961 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     7021 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     7081 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     7141 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     7201 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     7261 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     7321 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     7381 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     7441 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     7501 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     7561 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     7621 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7681 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     7981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8041 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     8101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8341 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     8401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     8881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     8941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     9541 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     9601 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     9661 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9721 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9781 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     9841 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     9901 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     9961 gga
//
